| 三株牛支原体的致病性比较 |
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| 引用本文： | 刘重阳,黄海碧,范文霞,乌吉斯古楞,张彦婷,陈学飞,纪燕,刘玉梅,宋庆庆,关平原.三株牛支原体的致病性比较[J].中国动物检疫,2021,38(2):114-119. |
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| 作者姓名： | 刘重阳 黄海碧 范文霞 乌吉斯古楞 张彦婷 陈学飞 纪燕 刘玉梅 宋庆庆 关平原 |
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| 作者单位： | 内蒙古农业大学兽医学院;金宇保灵生物药品有限公司 |
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| 摘 要： | 为检测从内蒙古发病牛场分离到的3株牛支原体(HS2019、HSZ2019、HSS2019)的致病性,对其进行本动物回归试验,通过观察攻毒后的临床症状、病理变化,以及应用实时荧光定量PCR确定组织器官中支原体载量,分析3株支原体的毒力。结果显示,3株牛支原体回归牛体后均使试验牛出现体温升高、咳嗽、呼吸困难等临床症状,解剖可观察到试验牛肺部损伤以及肺脏与胸腔粘连的病理变化,其中HS2019株较其他两株引起的症状与病变更为明显,组织脏器中的载菌量最高。结果表明,这3株支原体均有致病性,其中HS2019株致病性最强,可作为今后疫苗研制的预备菌株。本研究既为国内疫苗的研制提供了菌株资源,也为今后牛支原体的免疫攻毒试验提供了评价标准。
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| 关 键 词： | 牛支原体 动物回归试验 荧光定量PCR |
Comparison of the Virulence of Three Strains of Mycoplasma bovis |
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| Liu Chongyang,Huang Haibi,Fan Wenxi,Wujisiguleng,Zhang Yanting,Chen Xuefei,Ji Yan,Liu Yumei,Song Qingqing,Guan Pingyuan.Comparison of the Virulence of Three Strains of Mycoplasma bovis[J].China Journal Of Animal Quarantine,2021,38(2):114-119. |
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| Authors: | Liu Chongyang Huang Haibi Fan Wenxi Wujisiguleng Zhang Yanting Chen Xuefei Ji Yan Liu Yumei Song Qingqing Guan Pingyuan |
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| Institution: | (College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018,China;Jinyu Baoling Biopharmaceutical Co.,Ltd.,National Engineering Laboratory for Veterinary Vaccine,Hohhot,Inner Mongolia 010020,China) |
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| Abstract: | In order to test the virulence of three strains of Mycoplasma bovis(HS2019,HSZ2019 and HSS2019)isolated from an infected farm in Inner Mongolia,an animal regression test was carried out,the number of Mycoplasma in tissue and organs was determined by observing clinical symptoms and pathological changes after virus challenge as well as by real-time fluorescent quantitative PCR,then the virulence of the three strains was analyzed.The results showed that such clinical symptoms as increasing body temperature,cough and dyspnea appeared in experimental cattle after the regression test of the three strains.The lung lesions as well as adhesion between lung and thoracic cavity and other pathological changes could be observed after anatomy,especially HS2019 strain,by which more obvious symptoms and lesions were caused,and the volume of Mycoplasma in tissues was maximum.In conclusion,the three strains of Mycoplasma were all pathogenic,especially HS2019 strain whose virulence was the strongest,which could be used as the preparation strain for vaccine development in the future.By the study,strain resources were provided for the development of vaccines,and evaluation criterion for immune challenge test of Mycoplasma bovis in the future was also provided. |
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| Keywords: | Mycoplasma bovis animal regression test quantitative real-time PCR |
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