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| 鸟分支杆菌鸟亚种泛酸激酶原核表达及纯化 | |
| 引用本文： | 陈鸿军,王伟,刘佩红,王水明,丁铲.鸟分支杆菌鸟亚种泛酸激酶原核表达及纯化[J].动物医学进展,2010,31(1):13-16. |
| 作者姓名： | 陈鸿军 王伟 刘佩红 王水明 丁铲 |
| 作者单位： | 1. 中国农业科学院上海兽医研究所,上海,200241 2. 上海市动物疫病预防控制中心,上海,200102 3. 江苏省出入境检验检疫局,江苏南京,211000 |
| 基金项目： | 上海市科技兴农重点项目[沪农科攻字(2006)第5-4号] |
| 摘 要： | 为了寻找分支杆菌耐药菌的新药靶点,根据GenBank的相应序列,利用特异性引物经PCR扩增获得了鸟分支杆菌鸟亚种的泛酸激酶基因,将该基因亚克隆入pET32a(+)载体中,经IPTG诱导和SDS-PAGE检测。结果表明,该基因成功获得表达,并以可溶性蛋白形式存在,利用His纯化试剂盒获得了纯化产物,为下一步进行酶活性测定、酶活性位点的突变及合成酶类似物等研究奠定了基础。 |
| 关 键 词： | 鸟分支杆菌鸟亚种 泛酸激酶 可溶性表达 |
Protokaryonic Expression and Purification of coaA in M.avium subsp. avium |
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| CHEN Hong-jun,WANG Wei,LIU Pei-hong,WANG Shui-ming,DING Chan.Protokaryonic Expression and Purification of coaA in M.avium subsp. avium[J].Progress In Veterinary Medicine,2010,31(1):13-16. | |
| Authors: | CHEN Hong-jun WANG Wei LIU Pei-hong WANG Shui-ming DING Chan |
| Institution: | 1.Shanghai Veterinary Research Institute;CAAS;Shanghai;200241;China;2.Shanghai Municipal Center for Disease Control and Prevention;200102;3.Jingsu Provin Entry-Exit Inspection and Quarantine Bureaue;Nanjing;Jiangsu;211000;China |
| Abstract: | Mycobacteriosis avium is an important zoonosis caused by M.avium complex,especially by M.avium subsp.avium(Maa).With the development of strains of high drug resistance,research on new targets of drugs should be extremely urgent.Pantothenate kinase(or coaA) might be a potential target for new drugs.In current study,coaA gene of Maa was amplified and subcloned into pET32a(+) vector.With the transfected BL21(DE3) bacteria induced by IPTG,the gene was successfully expressed.And the product was soluble in E.coli... |
| Keywords: | Mycobacterium avium subsp avium coaA soluble expression |
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