Multiplex PCR method for differentiating highly pathogenic Yersinia
enterocolitica and low pathogenic Yersinia enterocolitica,and
Yersinia pseudotuberculosis |
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| Authors: | Thi Hien BUI Shunsuke IKEUCHI Yukiko SASSAO&#x;-BRIEN Takeshi NIWA Yukiko HARA-KUDO Takahide TANIGUCHI Hideki HAYASHIDANI |
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| Institution: | 1)Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan;2)National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-9501, Japan |
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| Abstract: | A multiplex PCR method for rapid and sensitive diagnosis, differentiating three pathogenic Yersinia groups such as the highly pathogenic Y. enterocolitica, including serotype O8, low pathogenic Y. enterocolitica, and Y. pseudotuberculosis, was developed. Four primer pairs were chosen to detect the genes fyuA, ail, inv, and virF, responsible for the virulence in pathogenic Yersinia species. Under the multiplex PCR conditions, the unique band patterns for the highly pathogenic Y. enterocolitica, low pathogenic Y. enterocolitica, and Y. pseudotuberculosis were generated from Yersinia strains. The detection limit of this method was 101–103 CFU per reaction tube. This multiplex PCR method could detect highly pathogenic Y. enterocolitica O8 from the wild rodent fecal samples that were culture-positive. Therefore, the new multiplex PCR method developed in this study is a useful tool for rapid and sensitive diagnosis, distinguishing three pathogenic Yersinia groups. |
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| Keywords: | detection diagnosis multiplex PCR Yersinia enterocolitica Yersinia pseudotuberculosis |
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