| 传染性法氏囊病病毒浙江分离株(TL2004)A节段cDNA的克隆及序列分析 |
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| 引用本文： | 郑江涛,魏永伟,刘岩,于涟.传染性法氏囊病病毒浙江分离株(TL2004)A节段cDNA的克隆及序列分析[J].中国预防兽医学报,2006,28(6):658-662,667. |
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| 作者姓名： | 郑江涛 魏永伟 刘岩 于涟 |
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| 作者单位： | 浙江大学,动物预防医学研究所浙江省重点实验室,浙江,杭州,310029 |
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| 基金项目： | 国家科技计划;浙江省科技计划 |
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| 摘 要： | 用Long accurate RT—PCR（LA-PCR）的方法一步法克隆了IBDV TL2004基因组A节段全长cDNA。序列测定结果表明：克隆的A节段全长3260个核苷酸，包括5＇、3＇的非编码区（NCRs）和2个重叠的开放阅读框（ORF1和ORF2），与来自GenBank IBDV血清Ⅰ型毒株核苷酸序列的同源性高达95．2％～99．2％。二级结构分析表明，在5＇-NCRs和3＇-NCRs各存在一个大型的颈环发夹结构。ORF1编码1012个氨基酸的VP2-VP3-VP4，在氨基酸水平上VP2、VP3、VP4与参比Ⅰ型毒株的同源性分别达96．7％～99．6％、94．2％～99．2％、96．7％～99．6％。ORF2编码145个氨基酸的VP5，与参比I型毒株的同源性高达95．9％～99．3％。TL2004在第2个小亲水区内279位氨基酸由S替代了N、290位M替代了L，这2个突变可能是IBDV抗原漂变的原因。分子系统进化树分析表明，TL2004与欧洲Cu21株和中国JD1、Harbin株的关系较近．而与欧洲、香港、日本的超强毒株和美国的变异株相对较远。
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| 关 键 词： | 基因组A节段全长cDNA 克隆 序列分析 |
| 文章编号： | 1008-0589（2006）06-0658-05 |
| 收稿时间： | 2005-08-12 |
| 修稿时间： | 2005-08-12 |
Clonging and sequence analysis of full-length segment A of infectious bursal disease virus(TL2004)isolated in Zhejiang province |
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| ZHENG Jiang-Tao,WEI Yong-Wei,Liu Yan,YU Lian.Clonging and sequence analysis of full-length segment A of infectious bursal disease virus(TL2004)isolated in Zhejiang province[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(6):658-662,667. |
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| Authors: | ZHENG Jiang-Tao WEI Yong-Wei Liu Yan YU Lian |
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| Institution: | Institute of Preventive Veterinary of Zhejiang University, Hangzhou 310029, China |
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| Abstract: | The full-length segment A of infectious bursal virus(TL2004)isolated from Zhejiang province was cloned and sequenced.The result showed that the segment A contains 3260 nucleotides including two overlapping open reading frames (ORF1 and ORF2)flanked by 5'-and 3'-noncoding regions(NCRs).ORF1 and ORF2 encoded VP5 of 145 amino acid residues and the polyprotein(VP2-4-3)of 1012 amino acid residues respectively.Compared with other stains from GenBank,TL2004 segment A shared 95.2% to 99.2% homology at nucleotide level.A hairpin structure was predicted in 5'-NCR and Y-NCR of the segement A.VP5 shared 95.9% to 99.3% homology with other strains.The deduced amino acids sequence of VP2,VP3, VP4 shared 96.7% to 99.6%,94.2% to 99.2%,97.1% to 99.2% homology with other strains respectively.The substitutions at 279(N to S)and 290(L to M)were responsible for the antigen variation.Phylogenetic tree analysis indicated TL2004 is more related to the European classical strains than those of vv IBDV and variant strains. |
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| Keywords: | IBDV |
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