Identification of <Emphasis Type="Italic">Pseudoperonospora cubensis</Emphasis> using real-time PCR and high resolution melting (HRM) analysis |
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| Authors: | Jeong?Hwan?Lee Myong?Ho?Park Email author" target="_blank">Sanghyeob?LeeEmail author |
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| Institution: | 1.Department of Bioresource Engineering and Plant Engineering Research Institute,Sejong University,Seoul,South Korea |
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| Abstract: | To develop a molecular method to identify Pseudoperonospora cubensis in cucumber leaves with signs of downy mildew, we compared the nucleotide sequences of ribosomal DNA-internal transcribed spacer, cytochrome oxidase II, and β-tubulin genes of P. cubensis and P. humuli isolates. Seven single nucleotide polymorphisms (SNPs) distinguished P. cubensis and P. humuli based on variations in β-tubulin sequences, and one specific primer set was designed for further analysis. Real-time PCR and high resolution melting analysis showed that the primer set can be used to specifically identify P. cubensis in cucumber leaves with downy mildew. |
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