| 中国广东家蚕微粒子孢子在Antheraea eucalypti细胞系的感染增殖 |
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| 引用本文： | 郑祥明,安勇智佐,河原烟勇.中国广东家蚕微粒子孢子在Antheraea eucalypti细胞系的感染增殖[J].蚕业科学,1999,25(3). |
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| 作者姓名： | 郑祥明 安勇智佐 河原烟勇 |
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| 作者单位： | 1. 广东省农业科学院蚕业研究所,广州,510642
2. 日本九州大学农学部 |
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| 摘 要： | 将中国广东的Ｎｏｓｅｍａ ｂｏｍｂｙｃｉｓ ＣＧＳ，用０２ｍｏｌ／ＬＫＯＨ 处理后，接种感染Ａ．ｅｕｃａｌｙｐｔｉ 细胞系。孢子的发芽率达８７％ ，在Ａ．ｅｕｃａｌｙｐｔｉ 细胞初期感染率为２７ ％ 。发育各阶段具２ 核，可观察到短极丝孢子和二次感染体形成，孢子芽母细胞按二分裂形成２ 个双核的孢子芽，具典型 Ｎｏｓｅｍａ 属特征。Ｎｏｓｅｍａｂｏｍｂｙｃｉｓ ＣＧＳ 生物学特性、血清学类型与日本Ｎｏｓｅｍａ ｂｏｍｂｙｃｉｓ ＮＩＳ００１ 大致相同，但孢子大小、裂殖体的形态、在Ａ．ｅｕｃａｌｙｐｔｉ 细胞寄生的细胞感染率、每个细胞的产孢数，２ 种微孢子虫却有差异。
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| 关 键 词： | 家蚕微粒子孢子 培养细胞 感染增殖 |
| 修稿时间： | 1998－09－14 |
Infection and Multiplication of Nosema bombycis From Silkworm of Guangdong, China in Antheraea eucalypti Cell Line |
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| Zheng Xiangming,Chisa Yasunaga,Takeshi Kawarabata.Infection and Multiplication of Nosema bombycis From Silkworm of Guangdong, China in Antheraea eucalypti Cell Line[J].Acta Sericologica Sinica,1999,25(3). |
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| Authors: | Zheng Xiangming Chisa Yasunaga Takeshi Kawarabata |
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| Abstract: | Nosema bombycis CGS separated from silkworms of Guangdong,China were treated with 0.2mol/L KOH solution and then inoculated in Antheraea eucalypti cell line.The percentage of hatched spores was 87% averagely;The infection rate at initial stage in A.eucalypti cell line was 2.7%.Binucleat could be found during the whole life cycle.Secondary infective form and few coiled spores could be seen.Sporont formed two binucleate sporoblasts through binary fission,which were consistent with the characteristics of Genus Nosema .The biological feature and serum type nearly accorded with N.bombycis NIS001 of Japan.But the size of the spore,the shape of the schizont,the rate of infection in A.eucalypti cell line,the number of spores laid in per cell were different between them. |
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| Keywords: | Nosema bombycis Cultured cell Infection and Multiplication |
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