| 甜菜M14品系BvM14-STPK基因的生物信息学分析及响应盐胁迫的表达分析 |
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| 引用本文： | 赵冬美,张咏雪,李海英.甜菜M14品系BvM14-STPK基因的生物信息学分析及响应盐胁迫的表达分析[J].中国农学通报,2020,36(1):35-42. |
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| 作者姓名： | 赵冬美 张咏雪 李海英 |
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| 作者单位： | 1. 黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨 150500;2. 黑龙江大学生命科学学院 黑龙江省普通高校分子生物学重点实验室,哈尔滨 150080 |
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| 基金项目： | 国家自然科学基金资助项目“甜菜M14 品系抗氧化酶系统响应盐胁迫应答过程的研究”(31471552)；黑龙江省高校创新团队建设计划项目 “寒区植物重要基因资源的挖掘与种质创新”(2014TD004)。 |
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| 摘 要： | 旨在进一步研究BvM14-STPK蛋白激酶对盐胁迫的应答反应,本研究以甜菜M14品系为材料,使用特异性引物通过聚合酶链式反应进行扩增,获得BvM14-STPK基因cDNA全长,并进行生物信息学分析,采用荧光定量PCR探究该基因响应盐胁迫的表达分析。生物信息学分析结果表明,BvM14-STPK基因cDNA全长1668 bp,包含1527 bp的最大ORF,编码508个氨基酸;BvM14-STPK蛋白激酶具有典型的蛋白激酶保守结构域。实时荧光定量PCR结果表明,BvM14-STPK基因在甜菜M14品系叶、根中均有广泛表达,叶中的表达量高于根中。该基因在200、400 mmol/L NaCl处理下,在叶片和根中呈现不同的表达状态,表明该基因可以应答盐胁迫,但在不同组织中应答盐胁迫的表达量存在差异。本项研究在挖掘甜菜M14品系优质基因和提高栽培甜菜抗逆性等方面具有重要指导意义,为进一步开展甜菜遗传改良工作奠定基础。
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| 关 键 词： | 甜菜M14品系 BvM14-STPK 生物信息学分析 荧光定量PCR 盐胁迫 |
| 收稿时间： | 2019/7/4 0:00:00 |
| 修稿时间： | 2019/8/29 0:00:00 |
Bioinformatics Analysis of BvM14-STPK Gene of Sugar Beet M14 Line and Expression Analysis of Its Response to Salt Stress |
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| Zhao Dongmei,Zhang Yongxue,Li Haiying.Bioinformatics Analysis of BvM14-STPK Gene of Sugar Beet M14 Line and Expression Analysis of Its Response to Salt Stress[J].Chinese Agricultural Science Bulletin,2020,36(1):35-42. |
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| Authors: | Zhao Dongmei Zhang Yongxue Li Haiying |
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| Institution: | 1. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500;2. Key Laboratory of Molecular Biology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080 |
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| Abstract: | We aim to gain insight to the BvM14-STPK protein kinase in response to salt stress. In this study, sugar beet M14 line was used as material, the full length cDNA of BvM14-STPK gene was amplified by RT-PCR with specific primers. Bioinformatics was used to analyze the full-length cDNA sequence and real-time fluorescence quantitative PCR was carried out to determine the expression patterns of BvM14-STPK in the sugar beet M14 line in response to salt stress. The results of bioinformatics analysis indicated that the full-length of BvM14-STPK has 1668bp with a 1527 bp ORF, the BvM14-STPK protein contains 508 amino acids. The BvM14-STPK contained a typical protein kinase conserved domain. The results of real-time fluorescence quantitative PCR showed that the BvM14-STPK gene was expressed in the leaves and roots , with leaves showing higher levels than roots. Under the 200 and 400 mmol/L NaCl stress, the gene showed different expression patterns in leaves and roots, indicating that the gene can resistance to salt stress, but there were ubiquitously expressed in different organs under salt stresses. This work has important significance for improving quality genes of sugar beet M14 line and enhancing crop salt-stress tolerance. It is a potential target for lay a foundation for further research on genetic engineering of sugar beet. |
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| Keywords: | sugar beet M14 line BvM14-STPK bioinformatics analysis fluorescence quantitative PCR salt stress |
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