| 藏药波棱瓜离体再生体系的建立 |
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| 引用本文： | 曾钰婷,刘正玉,卫华,李淑萍.藏药波棱瓜离体再生体系的建立[J].甘肃农业大学学报,2009,44(6). |
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| 作者姓名： | 曾钰婷 刘正玉 卫华 李淑萍 |
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| 作者单位： | 西藏自治区农牧科学院蔬菜研究所,西藏,拉萨,850032 |
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| 基金项目： | 西藏自治区财政专项资助 |
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| 摘 要： | 由于长期对波棱瓜野生资源的采挖,使其现已濒临灭绝,通过组培快繁技术进行繁殖是解决波棱瓜资源短缺的有效途径之一.本试验以波棱瓜的顶芽为外植体进行离体培养与植株再生的研究.结果表明,波棱瓜的继代增殖以MS+1.5 mg.L-16-BA+0.05 mg.L-1NAA为宜,每个外植体可平均产生4.5个芽;生根培养基以1/2MS+1.0 mg.L-1IAA为宜,生根率达96%.
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| 关 键 词： | 波棱瓜 离体培养 植株再生 |
Culture and plant regeneration of tibet medicine Herpetospermum peduncutosum in vitro |
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| ZENG Yu-ting,LIU Zheng-yu,WEI Hua,LI Shu-ping.Culture and plant regeneration of tibet medicine Herpetospermum peduncutosum in vitro[J].Journal of Gansu Agricultural University,2009,44(6). |
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| Authors: | ZENG Yu-ting LIU Zheng-yu WEI Hua LI Shu-ping |
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| Institution: | ZENG Yu-ting,LIU Zheng-yu,WEI Hua,LI Shu-ping(Institute of Vegetable Sciences,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lasa 850032,China) |
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| Abstract: | Herpetospermum peduncutosum is dying out because of continuously digging. It is an efficiency way to solve resource lack by rapid-propagation technology of tissue culture. Callus was obtained from the terminal bud explants of H. peduncutosum on MS medium supplemented. Following subculture on MS medium supplemented with 1.5 mg·L~(-1) 6-BA and 0.05 mg·L~(-1) NAA. Each explant produced 4.5 shoots. The frequency of rooting was 96 % on 1/2 MS medium supplemented with 1.0 mg·L~(-1) IAA . |
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| Keywords: | Herpetospermum peduncutosum in vitro plant regeneration |
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