| 提高小麦×玉米杂交产生单倍体胚频率的研究 |
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| 引用本文： | 王成社,闫林.提高小麦×玉米杂交产生单倍体胚频率的研究[J].农业生物技术学报,2007,15(2). |
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| 作者姓名： | 王成社 闫林 |
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| 作者单位： | 西北农林科技大学 |
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| 摘 要： | 为了提高小麦×玉米杂交单倍体胚的产生频率，采用了2,4-D中含有不同体积浓度的二甲基亚砜和不同的激素处理组合两种方法诱导小麦×玉米产生单倍体，研究其对得胚率的影响。结果表明，就平均得胚率而言，在二甲基亚砜激素浓度处理中，最佳的二甲基亚砜体积浓度为3.0%（得胚率为17.0%），而且各不同浓度的处理均高于对照（9.7%）；在不同激素配比试验中，2,4-D+3%二甲基亚砜单独处理的得胚率（7.3%）﹥2,4-D两次处理(6.9%)﹥2,4-D与2,4-D+3%二甲基亚砜配合处理(5.5%)﹥2,4-D一次处理(2.9%)。
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| 关 键 词： | 小麦 玉米 单倍体 2 4-D 二甲基亚砜 得胚率 |
| 收稿时间： | 2006-4-6 |
| 修稿时间： | 2006-7-2 |
Study on Increasing Frequency of Wheat Haploid Embryo Production through Wheat×Maize |
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| Abstract: | In order to increase the frequency of wheat haploid embryo formation through wheat × maize, we implied two method of hormone treated: Different volume concentrations of DMSO in 100mg/L 2, 4-D (treatment 1) and different hormone treatment combinations (treatment 2). The results showed that different volume concentrations of DMSO and different hormone treatment combinations had difference on the frequency of embryo formation. In treatment 1, the optimal volume concentration of DMSO in 2, 4-D is 3.0%(17.0%), moreover the frequencies of haploid embryo formation in other volume concentrations of DMSO are all higher than in control (9.7%).The frequency of embryo formation of different hormone treatment combinations was that 2,4-D+3.0% DMSO single treatment (7.3%)﹥2,4-D twice treatments (6.9%)﹥2,4-D and 2,4-D+3.0% DMSO combined treatment (5.5%)﹥2,4-D single treatment (2.9%). |
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