Effect of ERK1/2 on ischemic preconditioning in diabetic rat hearts |
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| Authors: | MA Cai-yun ZHANG Li-ke DU Feng-he LU Ling-qiao WANG Hong-xia ZENG Xiang-jun FU Ping MAN Qing-qing |
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| Institution: | 1.Department of Cardiology, Beijing Tiantan Hospital，Beijing 100050, China;2.Department of Pathophysiology, Capital University of Medical Sciences, Beijing 100054, China;3.Institute of Safety of Nutrition and Food, Chinese Center for Disease Control, Beijing 100050, China |
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| Abstract: | AIM: To test the effect of ERK1/2 on ischemic preconditioning (IPC) in diabetic rat hearts. METHODS: The diabetic rat model was made with alloxan. After eight weeks, 24 rats were divided into 4 groups: non-diabetic IPC rats (group A); non-diabetic non-IPC rats (group B); diabetic IPC rats (group C); diabetic non-IPC rats (group D). ECGⅡ lead, left ventricular development pressure (LVDP), and first derivative of LVDP (±dp/dtmax) were recorded. Myocardial phosphorylation of extracellular signal regulated kinases1/2 (ERK1/2) was detected by Western-blotting. RESULTS: (1) The ventricular arrythmia score was significantly lower in group A than that in group C (P<0.01). (2) LVDP and +dp/dtmax % were significantly higher in group A than those in group B and group C (P<0.01). -dp/dtmax% was significantly higher in group A than that in group B. (3) Phosphorylation of myocardial ERK1/2 was significantly higher in group A than those in group B and group C. CONCLUSIONS: IPC reduced the severity of ischemia/rerperfusion- induced arrhythmias, improved cardiac function in non-diabetic rats. However, such beneficial effects of IPC were not observed in diabetic rats. The attenuation of cardioprotection by IPC in diabetic animals may be due to the decrease in the activation of myocardial ERK1/2. |
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