Influence of laminin on the expression of bcl-2 in rabbit cornea endothelial cells |
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| Authors: | ZHU Xiao-qian WU Jing XU Jin-tang ZHAO Song-bin |
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| Institution: | Department of Ophthalmology, Medical College of Jinan University, Guangzhou 510632, China |
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| Abstract: | AIM: To investigate the influence of laminin (LN) on the expression of bcl-2 in rabbit cornea endothelial cells. METHODS: Cultured rabbit corneal endothelial cells were divided into three groups: normal control group, LN group and negative control group. Immunohistochemical technique and ELISA were used to measure the staining and A value to assess the levels of bcl-2 expression. RESULTS: Bcl-2 expression score showed that LN group had a strong positive expression. Control group only showed a weak expression. Negative control group showed a negative expression by immunohistochemical staining. The mean A value of each group were 1.21±0.18 (LN group)， 1.05±0.14 (normal control group) and 0.04±0.01 (negative control group). CONCLUSION: LN promotes bcl-2 gene expression in rabbit cornea endothelial cells， and protects the cells from apoptosis. |
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