Implantation study on the potential of murine epidermal stem cell differentiation |
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| Authors: | LIN Yi LI Hai-biao HUANG Jin-tao ZENG Shan DI Jing-fang ZENG Yao-ying |
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| Institution: | 1.Department of Histology and Embryology, Medical College of Zhongshan University Guangzhou 510080, China;2.Key Laboratory of Ministry of Education for Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China. E-mail: yilinonline@21cn.com |
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| Abstract: | AIM: To investigate the potential of murine epidermal stem cell (ESC) differentiation after seeded in a biodegradable carrier and implanted subcutaneously into syngeneic recipient mice. METHODS: ES cells were induced in vitro to differentiate into ESCs. After stained with a fluorescent dye Hoechst 33342, these ESCs were seeded into a polyglycolic acid (PGA) net containing collagen gel, functioning as a cell carrier, and implanted subcutaneously into 129/J mice, which were syngeneic to these stem cells. RESULTS: The ESCs kept alive in the implant when observed under a fluorescent microscopy 3 weeks or longer after implantation, and could differentiate into hair follicle-like structure, glandular structure, and gave rise to additional structures displaying features resembling native dermis. No apparent rejection or severe side effects were observed at least 10 weeks post-implantation. CONCLUSION: It is feasible to use these ESCs as seed cells in the study to fabricate dermal equivalent having the potential to develop dermal appendages. |
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| Keywords: | Cell surface marker Epidermis Stem cells |
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