The relationship between high density lipoprotein subclasses and apoE gene polymorphism in obese subjects |
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| Authors: | LONG Shi-yin XU Yan-hua FU Ming-de ZHANG Xue-mei TIAN Ying LIU Bing-wen |
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| Institution: | 1.Department of Biochemistry, West China School of Preclinicl and Forensic Medicine, Sichuan University, Chengdu 610041, China;2.Hoist Group Postdoctoral Work Station, Chengdu 610041, China;3.Department of Biochemistry and Molecular Biology, Nanhua University, Hengyang 421001, China |
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| Abstract: | AIM: The purpose of this study is to elucidate the relationship between apolipoprotein (apo) E polymorphism and plasma lipid profiles and HDL subclasses in obesity. METHODS: apoE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 93 obese subjects and 96 nonobese subjects were determined by two-dimensional gel electrophoresis conjunction with immunodetection method. RESULTS: apoE3/3 genotypes and allele ε3 frequency in obese group and the control group were both the highest, but no significant difference. In obese group, the genotype of apoE2 had higher serum apoE/aopCⅢ, HDL2a and lower apoB100, apoCⅢ, HDL3c levels compared with the genotype of apoE3 (P<0.05). In control group, the genotype of apoE2 had higher serum TC and apoE levels, but lower HDL3b level compared with the genotype of apoE3 (P<0.05). CONCLUSION: Polymorphism of the ApoE gene is associated with the distribution of HDL particles in obesity. Allele of ε2 carrier may slow the tendency of HDL particals shifted towards smaller sizes. |
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