Effects of angiotensin Ⅳ on growth of cultured neonatal rat cardiomyocytes |
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| Authors: | CHEN Min YANG Bo |
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| Institution: | Division of Cardiology, The Renmin Hospital of Wuhan University, Wuhan 430060, China |
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| Abstract: | AIM: To study the effects of angiotensin Ⅳ (Ang Ⅳ) on growth of cultured neonatal rat cardiomyocytes. METHODS: Cardiomyocytes isolated from neonatal SD rat were cultured in vitro and divided into groups: control DMEM, 10-6 mol/L Ang Ⅳ, 10-5 mol/L Ang Ⅳ. The effects of Ang Ⅳ were assayed as follows: protein synthesis was determined by modified Bradford method, and cell cycle by flow cytometry. RESULTS: Ang Ⅳ promoted protein synthesis (P<0.05), when cardiomyocytes were incubated for 36 h and 48 h, Ang Ⅳ promoted protein synthesis more evidently (P<0.01). Ang Ⅳ accelerated the change of rat cardiomyocytes from G0/G1 to S phases and increased the numbers of cells of S and G2/M phases. Besides, 10-6 mol/L AngⅣ accelerated the change of that from G0/G1 to S phases more evidently. CONCLUSION: Ang Ⅳ directly increases protein synthesis in cardiomyocytes in a time-dependent manner. Ang Ⅳ influences growth of cardiomyocytes in a concentration-dependent fashion. |
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