Apoptosis of colorectal cancer cells transfected with human mutant p27 |
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| Authors: | CHEN Jun XU Shao-yong XIONG Ling WANG Jia-ning HUANG Yong-zhang |
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| Institution: | 1.Department of Gastroenterology, Remin Hospital,2Institute of Clinical Medicine, Yunyang Medical College, Shiyan 442000, China |
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| Abstract: | AIM: To study the inducing effect of human mutant p27 gene on apoptosis of the colorectal cancer cells. METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the colorectal cancer cell SW480. The inducing effect of Ad-p27mt on apoptosis in colorectal cancer cells was measured by flow cytometry, DNA fragment analysis and TUNEL method. RESULTS: Ad-p27mt was successfully constructed. When the multiplicity of infection (MOI) was ≥50, the infection efficiency reached 100%. After 24 h of infection, there was an apoptotic hypodiploid peak observed by flow cytometry before G1 and there were apoptotic characteristic bands in the DNA electrophoresis. The apoptotic index detected by TUNEL method was 82.6±3.2 (Ad-p27mt group) and 5.0±3.5 (control group), respectively, the difference of which was significant (P<0.01). CONCLUSION: Human mutant p27 gene transfection effectively induces apoptosis in the colorectal cancer cells. |
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