Characteristics of eight established human melanoma cell lines |
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| Authors: | ZHU He-cheng WU Shang-hui HUANG Bai-ying GU Huan-hua LUO Xue-bin PENG Cong ZHU Bin |
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| Institution: | Laboratory of Cell Biology, Central South University, Changsha 410078, China |
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| Abstract: | AIM: Eight melanoma cell lines were established from human melanoma specimen and designated as HME 1-HME 8, and their characteristics of cell biology and immunology in vitro were studied. METHODS: The tissues from biopsies of human melanoma were cultured in RPMI 1640 media. After growing to 90% confluence, the cells were detached for subculture and then their characteristics, including morphology, growth kinetic, tumor antigen and tumorigencity in nude mice were studied. RESULTS: These cell lines have been passaged more than 100 times within 2 years. Their characteristics demonstrated: ① The population doubling time calculated in the log phase of growth were 34.2-59.5 h. ② The cloning efficiency in soft agar averaged 8.6%-26.2%. ③ The melanoma cells transplanted in nude mice were high tumorigencity. ④ Karyo-type analysis showed that aneuploidy with the modal chromosomal number 64-117. ⑤ Rich ribosomes and melanoid grains in the cytoplasm were observed under electronic microscope. ⑥ Immunohistochemistry analysis showed that there were a lot of brown grains in the cytoplasm. ⑦ Detection of tumor-antigen demonstrated that melan-A antigen was 75.0%, MAGE-1 was 50.0%, MAGE-2 was 37.5%, MAGE-3 was 62.5%, respectively, for eight melanoma cell lines. CONCLUSION: The melanoma cells have immortalized after being cultured in vitro and has specific characteristics of malignant melanoma. |
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