Conifer genetic engineering: Particle bombardment andAgrobacterium-mediated gene transfer and its application in future forests |
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| Authors: | Tang Wei |
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| Institution: | (1) Forest Biotechnology Group, North Carolina State University, Centennial Campus, P.O. Box 7247, 27695-7247 Raleigh, NC, USA |
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| Abstract: | Many important advances in forest biotechnology have been made. The use of genetic transformation and the applications of
transgenic trees in modern forestry is now an important field. Two basic methodologies particle bombardment andAgrobacterium-mediated transformation have been used on conifers. However, routine procedures exist for only a limited number of conifers.
As a result only a few species have been successfully transformed into stable transgenic plants. The use of a particle bombardment
has been more successful and transgenic plants have been produced inPicea abies, Picea glauca, Picea mariana, andPinus radiata, although the level of production of stable transgenic plants is lower than that ofAgrobacterium. At present, breeding programs have been directed toward improving bole shape, growth rate, wood properties, and quality,
as well as toward improving root and shoot performance, pest resistance, stress tolerance, herbicide resistance, and ability
to resist stresses, which will drive forestry to enter a new era of productivity and quality. This article provides a brief
overiew of the current state of knowledge on genetic transformation in conifers.
Biography: Tang Wei (1964-), male, Ph. Doctor, Research associate, O-23, E. S. King Village, 2900, Ligon St., North Carolina State
University, Raleigh, NC 27607, USA
Responsible editor: Chai Ruihai |
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| Keywords: | Genetic engineering Particle bombardment Agrobacterium Conifer |
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