| 利用分子标记EPT11_(900)辅助甘蓝显性雄性不育基因转育 |
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| 引用本文： | 王晓武,方智远.利用分子标记EPT11_(900)辅助甘蓝显性雄性不育基因转育[J].中国蔬菜,1998,1(6):1-0. |
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| 作者姓名： | 王晓武 方智远 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所; |
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| 摘 要： | 利用与甘蓝显性雄性不育基因（Ｍｓ）连锁的延长随机引物扩增ＤＮＡ（ＥＲＰＡＤ）标记ＥＰＴ１１９００对３３个甘蓝自交系的多态性进行了分析。ＥＰＴ１１９００主要分布在各种早熟甘蓝中，很少存在于鸡心类型或晚熟扁圆类型甘蓝中。该结果表明：它可用于辅助大多数鸡心型或晚熟扁圆类型甘蓝的Ｍｓ基因转育；ＥＰＴ１１９００在辅助两个甘蓝自交系回交三代及两个青花菜自交系回交一代的Ｍｓ基因转育中，预测的准确性超过９０％。
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| 关 键 词： | 甘蓝 显性雄性不育 延长随机引物扩增DNA标记（ERPAD） 辅助选择 |
| 收稿时间： | 1900-01-01; |
Assisted Transgression of Dominant Male Sterile Gene (Ms) in Cabbage by Using Molecular Marker EPT11 900 |
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| Wang Xiaowu,Fang Zhiyuan,Sun Peitian,et al..Assisted Transgression of Dominant Male Sterile Gene (Ms) in Cabbage by Using Molecular Marker EPT11 900[J].China Vegetables,1998,1(6):1-0. |
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| Authors: | Wang Xiaowu Fang Zhiyuan Sun Peitian |
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| Institution: | Institute of Vegetables and Flowers; Chinese Academy of Agricultural Sciences; Beijing 100081 |
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| Abstract: | Polymorphism was analyzed in 33 inbred lines of cabbage by using an extended random primer amplified DNA (ERPAD) marker EPT11 900 linked to a dominant male sterile gene in cabbage. The results indicated that EPT11 900 mainly distributed among inbred lines with early maturity and rarely existed in inbred lines with chicken-hearted head or late flat head. It was feasible to employ EPT11 900 in marker assisted transgression of Ms gene to most lines with late maturity or with chicken-hearted head. Predicted precision was more than 90% when EPT11 900 was used to assist transgression of Ms gene in BC3 generation of two cabbage lines and BC1 generation of one broccoli lines. |
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| Keywords: | Cabbage ( Brassica oleracea var capitata ) Dominant male sterile gene Extended random primer amplified DNA (ERPAD) marker Assisted selection |
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