| 污染的霉形体在传代细胞内增殖的观察 |
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| 引用本文： | 杨盛华,武银莲,邹啸环,常国权.污染的霉形体在传代细胞内增殖的观察[J].中国兽医学报,1991(3). |
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| 作者姓名： | 杨盛华 武银莲 邹啸环 常国权 |
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| 作者单位： | 兽医大学军事兽医研究所
(杨盛华，武银莲，邹啸环)，兽医大学军事兽医研究所(常国权) |
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| 摘 要： | 对霉形体进入DK传代细胞内,并进行增殖的情况,作了详细的电镜观察.结果如下:(1)霉形体靠近传代细胞膜表面呈串珠样排列;霉形体与传代细胞膜表面接触部位融合增厚,电子密度增高,向内凹陷,将霉形体裹入细胞质,形成由膜结构包着的包涵体.(2)在传代细胞质中,除可见由膜结构包裹着的霉形体性包涵体外,还可见没有任何膜包着的散在的霉形体,并且均可见到霉形体的裂殖增殖相和出芽增殖相.(3)在传代细胞核的核周池和核质中,均见到典型的霉形体及其裂殖增殖相.(4)霉形体在传代细胞中大量的增殖,致使细胞崩解,霉形体即被释放出来.(5)霉形体使传代细胞出现严重的超微病变.本文还讨论了传代细胞培养污染霉形体后难以救治的根本原因是,霉形体在传代细胞内增殖,故仅仅着眼于消除培养液中的霉形体是不能达到预期结果的.
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| 关 键 词： | 霉形体 传代细胞 细胞内增殖 |
Observations on Intracellular Multiplication of Contaminated My- coplasmas in Dog Kidney Cell Line |
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| Yang Shenghua et al.Observations on Intracellular Multiplication of Contaminated My- coplasmas in Dog Kidney Cell Line[J].Chinese Journal of Veterinary Science,1991(3). |
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| Authors: | Yang Shenghua |
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| Abstract: | The multiplication of contaminated mycoplasmas in the cytoplasm and nucleus of dog kidney cell line (used as culture control) was observed by electron microscopy when cell culture work was performed for other purpose. The observable feature were as follows: (1) The mycoplasmas formed a structure like a string of beads adjacent to the cell membrane and fused with it , the fused region became thick and higher electron dense and recessed to enclose the mycoplas-ma resulting in the formation of an inclusion; (2) additionally, there were some unclosed mycoplasmas disseminating within the cytoplasm and they exhibited division and budding; (3) the intracellular mycoplasmas multiplied in perinuclear cisternae and nuclein of the nuclei; (4) the multiplied mycoplasmas were then released into extracellular culture medium after cell destruction due to they plenty multiplied; (5) the serious ultrastructural lesions of the infected cells were demonstrated. It suggested that this is the reason why the mycoplasma contamination of the cell line could not easily be cleared away. |
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| Keywords: | mycoplasma intracellular mutiplication dog kidney cell line |
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