Identifying the alien chromosomes in wheat – Leymus multicaulis derivatives using GISH and RFLP techniques |
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| Authors: | Jizeng Jia Ronghua Zhou Pei Li Maolin Zhao Yushen Dong |
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| Institution: | (1) Institute of Crop Germplasm Resources, CAAS, Key Laboratory of Crop Germplasm & Biotechnology, Beijing, 100081, China |
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| Abstract: | Genomic in situ hybridization (GISH) and restriction fragment length polymorphism (RFLP) were used to identify the Leymus multicaulis (XXNN, 2n = 28) chromosomes in wheat-L. muliticaulis derivatives. Fifteen lines containing L. multicaulis alien chromosomes or chromosomal fragments were identified. All alien chromosomes or fragments in these 15 lines were from
the X genome and none were from the N genome. Eleven L. multicaulis disomic addition lines and four translocation-addition lines were identified with chromosome rearrangements among homoeologous
groups 2, 3, 6 and 7. Only homoeologous group 1 lacked rearrangements in addition or translocation chromosomes. The results
revealed that translocation in non-homoeologous chromosomes widely exists in the Triticeae and therefore it is necessary to
identify the alien chromosomes
(segments) in a wheat background using these combined techniques. During the course of the work, probe PSR112, was found to
detect X genome addition lines involving L. multicaulischromosomes. This may prove to be a valuable probe for the identification of alien chromosomes in a wheat background.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | in situ hybridization Leymus multicaulis RFLP wheat |
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