| 响应面法优化超声波循环提取长裙竹荪抗氧化物质工艺研究 |
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| 引用本文： | 王宏雨.响应面法优化超声波循环提取长裙竹荪抗氧化物质工艺研究[J].食用菌学报,2012,19(1):56-60. |
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| 作者姓名： | 王宏雨 |
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| 作者单位： | 福建省农业科学院食用菌研究所,福建福州,350014 |
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| 基金项目： | 福建省农业科学院青年基金项目和创新团队重点项目 |
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| 摘 要： | 利用Box-Benhnken中心组合实验和响应面分析法,在前期单因素试验基础上,以提取温度、超声功率和工作间歇比为自变量,以粗提物对DPPH.清除率为响应值,对超声波循环提取长裙竹荪(Dictyophoraindusiata)抗氧化物质工艺进行优化。优化后确定的最佳提取工艺条件为:提取温度45℃,超声功率580 W,工作间歇比3.6/1,在该条件下,粗提物对DPPH.自由基清除率达到46.45%,粗提物得率为30.37%。
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| 关 键 词： | 长裙竹荪 抗氧化活性 超声波循环提取 响应面法 |
Optimization of Ultrasound-Assisted Extraction of Antioxidant Components from Dictyophora indusiata Using Response Surface Methodology |
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| WANG Hongyu.Optimization of Ultrasound-Assisted Extraction of Antioxidant Components from Dictyophora indusiata Using Response Surface Methodology[J].Acta Edulis Fungi,2012,19(1):56-60. |
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| Authors: | WANG Hongyu |
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| Institution: | WANG Hongyu(Institute of Edible Fungi,Fujian Academy of Agriculture Sciences,Fuzhou,Fujian 350014,China) |
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| Abstract: | Selected parameters affecting ultrasound-assisted extraction of antioxidant components from Dictyophora indusiata fruit bodies were optimized using response surface methodology.Antioxidant activity was assessed by measuring the DPPH·scavenging effect of crude extracts.Under optimal extraction conditions(extraction temperature 45 ℃,ultrasonic power 580 W,ratio of treatment and intermittent times 3.6/1),the DPPH· scavenging rate of crude extracts(obtained in 30.4% yield) was 46.5% under the assay conditions. |
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| Keywords: | Dictyophora indusiata antioxidant activity ultrasound-assisted extraction response surface methodology |
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