| 中国三省松口蘑遗传多样性分析 |
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| 引用本文： | 马银鹏,陈强,赵梦然,邬向丽,张金霞,黄晨阳.中国三省松口蘑遗传多样性分析[J].食用菌学报,2012,19(1):17-26. |
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| 作者姓名： | 马银鹏 陈强 赵梦然 邬向丽 张金霞 黄晨阳 |
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| 作者单位： | 中国农业科学院农业资源与农业区划研究所,农业部农业微生物资源收集与保藏重点实验室,北京100081 |
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| 摘 要： | 采用ITS、mt SSU rDNA的V4和V9区对采自西藏、吉林和云南3个省份30个地区的59个样本进行物种鉴定,并运用IGS1-RFLP和RAPD进行遗传多样性分析。结果表明:所有样本均为松口蘑(Tricholoma matsutake),松口蘑ITS序列存在明显的地域性;IGS1-RFLP分析表明,吉林样本属于A型,西藏和云南样本是2种新类型;聚类分析表明,在相似系数为0.702水平时将供试样本分为3大类群;我国松口蘑具有丰富的遗传多样性,并且自然种群遗传多样性地域性明显。
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| 关 键 词： | 松口蘑 mtSSU IGS1-RFLP RAPD 遗传多样性 地理分布 |
Genetic Diversity of Chinese Tricholoma matsutake Strains from Tibet, Jilin and Yunnan Provinces |
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| MA Yinpeng
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CHEN Qiang
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ZHAO Mengran
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WU Xiangli
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ZHANG Jinxia
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HUANG Chenyang.Genetic Diversity of Chinese Tricholoma matsutake Strains from Tibet, Jilin and Yunnan Provinces[J].Acta Edulis Fungi,2012,19(1):17-26. |
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| Authors: | MA Yinpeng CHEN Qiang ZHAO Mengran WU Xiangli ZHANG Jinxia HUANG Chenyang |
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| Institution: | *(Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences;Key Laboratory of Microbial Resources,Ministry of Agriculture,Beijing 100081,China) |
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| Abstract: | Fifty nine mushroom samples were collected from 30 locations in Tibet,Jilin and Yunnan and identified as Tricholoma matsutake based on ITS sequences,and the V4 and V9 domains of the mitochondrial small-subunit(SSU).IGS1 markers were region-specific in that samples from a single province had the same sequence,whereas these sequences differed from the IGS1 sequences of samples originating from the other two provinces.Abundant genetic diversity among T.matsutake strains in China was revealed by RAPD analysis.Mushroom samples from Jilin corresponded to the previously designated type A,whereas two new types were observed among samples from Tibet and Yunnan.UPGMA-based clustering separated the 59 test strains into three groups at an overall similarity coefficient of 0.702. |
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| Keywords: | Tricholoma matsutake mt SSU IGS1-RFLP RAPD genetic diversity geographical distribution |
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