Effects of growth regulators and activated charcoal on in vitro bulblet multiplication and growth in Galanthus nivalis ‘Flore Pleno’ |
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| Authors: | I Staikidou |
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| Institution: | School of Biological Sciences, The Queen’s University of Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, UK |
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| Abstract: | SummaryThe in vitro multiplication of Galanthus nivalis ‘Flore Pleno’ bulblet clumps was evaluated through three 16-week sub-culture passages on media supplemented with either 1.0 mg l–1 6-benzylaminopurine (BA) and 0.1 mg l–1 naphthaleneacetic acid NAA; the plant growth regulator (PGR) control], 0.5 mg l–1 BA and 0.05 mg l–1 NAA (PGR/2), or 0.1 mg l–1 BA and 0.01 mg l–1 NAA (PGR/10). At the end of the second sub-culture passage, clumps of bulblets from each of the three PGR treatments were also transferred to conditions to promote bulblet growth (G medium without PGRs) supplemented with 60 g l–1 sucrose and 5 g l–1 activated charcoal (AC). Lowering the PGR concentration during the initial multiplication phase reduced bulblet multiplication and overall culture growth, but did not influence bulblet size, as indicated by the diameter of the largest bulblet. Lowering the PGR concentration during the multiplication phase also reduced the multiplication of bulblets, overall tissue fresh weight, and the production of roots in tissues transferred to bulblet growth conditions. The reduced growth of tissues previously multiplied on PGR/10 medium was not, however, reflected in the growth of individual bulblets, since these were not significantly smaller than bulblets initially multiplied at the higher PGR concentrations. Bulblet sprouting and root growth on the bulblet growth medium were unaffected by prior PGR status in the multiplication phase. The results are discussed in terms of the mode of action of AC on the promotion of bulblet growth. |
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