Rapid micropropagation of Psoralea corylifolia L. using nodal explants cultured in organic additive-supplemented medium |
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| Authors: | P Baskaran N Jayabalan |
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| Institution: | 1. Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalliy 620 024, Tamil Nadu, Indiabaskaran_research@yahoo.co.in;3. Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalliy 620 024, Tamil Nadu, India |
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| Abstract: | SummaryThe influence of different growth regulators and additives on shoot multiplication from nodal explants of Psoralea corylifolia was investigated. Prolific shoot multiplication was achieved within 4 weeks of culture on Murashige and Skoog (MS) medium supplemented with 5 μM benzyladenine (BA), 5 μM ascorbic acid (AA), 100 mg l–1 casein hydrolysate (CH) and 5% (v/v) coconut water (CW). Shoots elongated on half-strength MS basal medium devoid of inositol, but containing 5 μM 2-isopentenyladenine (2iP), 10 g l–1 sucrose and 8 g l–1 agar. Elongated shoots rooted on half-strength MS basal medium supplemented with 3 μM indole-3-butyric acid (IBA), 10 g l–1 sucrose and 7 g l–1 agar within 5 d of culture. The in vitro-raised plants were established successfully in 2:1:1 (v/v/v) garden soil:farmyard soil:sand, and maintained in a growth chamber with 100% survival. Acclimatised plants were transferred to a glasshouse and established successfully in the field. Flowers and fruits appeared after 4 months and resembled those on source plants. This system could be used for rapid commercial propagation of P. corylifolia for conservation strategies and to produce phytomedicines. |
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