| 猕猴桃果实L-半乳糖内酯脱氢酶和脱氢抗坏血酸还原酶cDNA片段的克隆与序列分析 |
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| 引用本文： | 牛歆雨,雷玉山,梁 东.猕猴桃果实L-半乳糖内酯脱氢酶和脱氢抗坏血酸还原酶cDNA片段的克隆与序列分析[J].西北农林科技大学学报(社会科学版),2007,35(12):57-62. |
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| 作者姓名： | 牛歆雨 雷玉山 梁 东 |
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| 作者单位： | 1. 西北农林科技大学,园艺学院,陕西,杨凌,712100
2. 陕西省中华猕猴桃科技开发公司,陕西,西安,710054 |
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| 基金项目： | 陕西省重大科技专项计划;西北农林科技大学校科研和教改项目 |
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| 摘 要： | 克隆猕猴桃果实L-半乳糖内酯脱氢酶(GalLDH)和脱氢抗坏血酸还原酶(DHAR)基因,可为揭示猕猴桃高抗坏血酸(AsA)含量的分子机制奠定基础。研究以猕猴桃果实为材料提取总RNA,采用RT-PCR法,克隆出L-GalLDH基因的cDNA片段857 bp和DHAR基因的cDNA片段594 bp并测序,根据测序结果推测其氨基酸序列,并与刺梨、拟南芥、花椰菜、甘薯和草莓等植物的GalLDH基因和DHAR基因氨基酸序列进行亲缘分析。结果表明,猕猴桃GalLDH基因氨基酸序列和核苷酸序列与刺梨的相似性高达88%,其在同源基因进化关系树中单独聚为一类;DHAR基因氨基酸序列和核苷酸序列与苹果的相似性高达98%和99%,两者在进化关系上聚为一类。可知克隆到的2个核苷酸片段确为猕猴桃L-GalLDH和DHAR基因cDNA片段,同源基因植物亲缘关系聚类与传统的形态分类有一定的差异。
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| 关 键 词： | 猕猴桃 L-半乳糖内酯脱氢酶(GalLDH) 脱氢抗坏血酸还原酶(DHAR) 维生素C 基因克隆 |
| 文章编号： | 1671-9387(2007)12-0057-06 |
| 收稿时间： | 2006/12/6 0:00:00 |
| 修稿时间： | 2006年12月6日 |
Cloning and sequence analysis of cDNA fragments of L-galactono-1,4-lactone dehydrogenase and dehydroascorbate reductase from fruit of Actinidia deliciosa |
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| NIU Xin-yu,LEI Yu-shan,LIANG Dong,MA Feng-wang,WANG Xi-rui.Cloning and sequence analysis of cDNA fragments of L-galactono-1,4-lactone dehydrogenase and dehydroascorbate reductase from fruit of Actinidia deliciosa[J].Journal of Northwest Sci-Tech Univ of Agr and,2007,35(12):57-62. |
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| Authors: | NIU Xin-yu LEI Yu-shan LIANG Dong MA Feng-wang WANG Xi-rui |
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| Institution: | 1 College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China;2 Shaanxi Province Zhonghua Kiwifruit Science and Technology Development Company,Xi’an,Shaanxi 710054,China) |
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| Abstract: | In order to research the molecular mechanism of Vc accumulation,857 bp and 594 bp cDNA encoding L-galactono-1,4-lactone dehydrogenase(GalLDH)and dehydroascorbate reductase(DHAR) fragment were cloned from fruit of Actinidia deliciosa by the method of RT-PCR,and then translated into amino acid sequences.In GenBank compared with Rosa roxburghii Trat,Arabidopsis thaliana,Ipomoea batatas.et.homologous genes plants,the nucleotide sequence and the amino acid sequence of GalLDH gene showed 88% of identity with those of Rosa roxburghii Tratt.The nucleotide sequence and the amino acid sequence of DHAR gene showed 98% and 99% of identity with those apples,respectively,and they both were classified the same family.These results suggest that the both cDNA fragments are from DHAR and GalLDH gene of Actinidia deliciosa. |
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| Keywords: | kiwifruit L-galactono-1 4-lactone dehydrogenase(GalLDH) dehydroascorbate reductase(DHAR) ascorbic acid(AsA) gene cloning |
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