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番茄种质资源亲缘关系的SSR分析
引用本文:丘漫宇,张素平,郭爽,李伯寿,刘玉平.番茄种质资源亲缘关系的SSR分析[J].中国蔬菜,2012,1(24):39-42.
作者姓名:丘漫宇  张素平  郭爽  李伯寿  刘玉平
作者单位:广州市农业科学研究院,广东广州 510308
基金项目:广州市科信局应用基础研究专项(2009J1-C171)
摘    要:采用25对SSR特异引物对20份番茄材料进行分析,其中21对引物扩增出条带,18对引物具有多态性,扩增出的66条谱带中有52条具有多态性,多态率为78.8%。遗传距离分析结果表明:20份番茄材料两两间的遗传距离(GD)在1.489 2~9.180 6之间,遗传距离大于7的育种材料共有18份,其中No.1与No.15之间的遗传距离最大,为9.180 6。以遗传相似系数0.67为标准可将20份番茄育种材料划分为4大类。

关 键 词:番茄  种质资源  亲缘关系  SSR  

Analysis of Tomato Germplasm Phylogenetic Relationship Using SSR Markers
QIU Man-yu , ZHANG Su-ping , GUO Shuang , LI Bo-shou , LIU Yu-ping.Analysis of Tomato Germplasm Phylogenetic Relationship Using SSR Markers[J].China Vegetables,2012,1(24):39-42.
Authors:QIU Man-yu  ZHANG Su-ping  GUO Shuang  LI Bo-shou  LIU Yu-ping
Institution:Guangzhou Academy of Agricultural Sciences,Guangzhou 510308,Guangdong,China
Abstract:25 SSR primers were used to study the genetic diversities among 20 accesions of tomato(Lycopersicon esculentum Mill.)germplasm resources detected from 66 bands.There were 52 bands which had polymorphism,and the polymorphism rate was 78.8%.The results of genetic distance analysis showed that the genetic distance of 20 tomato materials were 1.489 2-9.180 6.There were 18 tomato materials which the genetic distance was more than 7,No.1 and No.15 had the largest genetic distance.That was 9.180 6.The result of cluster analysis showed that 20 tomato germplasm resources could be classified into 4 groups.
Keywords:Germplasm resource  Phylogenetic relationship  SSR marker
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