| 斑节对虾性腺差异表达基因的筛选 |
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| 引用本文： | 陈亮,黄建华,张殿昌,苏天凤,江世贵.斑节对虾性腺差异表达基因的筛选[J].水产学报,2010,34(1):47-55. |
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| 作者姓名： | 陈亮 黄建华 张殿昌 苏天凤 江世贵 |
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| 作者单位： | 1. 中国水产科学研究院南海水产研究所,农业部海水养殖生态与质量控制重点开放实验室,广东,广州,510300;广东海洋大学水产学院,广东,湛江,524088
2. 中国水产科学研究院南海水产研究所,农业部海水养殖生态与质量控制重点开放实验室,广东,广州,510300 |
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| 基金项目： | 国家自然科学基金项目(NSFC40976101); 国家“八六三”高技术研究发展计划(2006AA10A406); “十一五”国家科技支撑计划(2006BAD01A13-2); 公益性行业(农业)科研专项(nyhyzx07-042); 广东省自然科学基金项目(7003894); 广东省科技计划项目(2006A20204001,2007A020400001) |
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| 摘 要： | 以池塘养殖250日龄的斑节对虾性腺组织为材料,利用mRNA差异显示(differential display,DD)筛选性腺差异表达基因,获得173条雌、雄性腺差异表达片段。随机选取44条差异片段进行回收、克隆、测序及Real-timeRT-PCR验证,最终获得10条阳性差异表达片段,其中OA7-1、OA7-2、OG8-1、OG8-2、OC1-3、OC4-2、OC8在卵巢中表达量极显著(P<0.01)高于精巢,TG6-3、TG8在精巢中表达量极显著(P<0.01)高于卵巢,TC1为精巢特异表达片段。序列分析表明OA7-2为脱氧尿嘧啶三磷酸核苷酸水解酶(dUTPase)(E值6e-54);OG8-1为真核细胞翻译起始因子3亚单位4(eIF-3delta)(E值9e-58);OC1-3为未命名蛋白产物(unnamed protein product)(E值2e-17);OC4-2为60S核糖体蛋白L7(RPL7)(E值6e-40);OC8为60S核糖体蛋白L3(RPL3)(E值5e-36);OA7-1、OG8-2和TC1与BLASTx比对同源性较低(10-4
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| 关 键 词： | 斑节对虾 性腺 差异显示 差异片段 |
| 收稿时间： | 2009/2/12 0:00:00 |
| 修稿时间： | 2009/6/18 0:00:00 |
Screening of differentially expressed genes of gonads from the pond-reared black tiger shrimp(Penaeus monodon) |
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| CHEN Liang,HUANG Jian-hu,ZHANG Dian-chang,SU Tian-feng and JIANG Shi-gui.Screening of differentially expressed genes of gonads from the pond-reared black tiger shrimp(Penaeus monodon)[J].Journal of Fisheries of China,2010,34(1):47-55. |
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| Authors: | CHEN Liang HUANG Jian-hu ZHANG Dian-chang SU Tian-feng and JIANG Shi-gui |
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| Institution: | CHEN Liang1,2,HUANG Jian-hua1,ZHANG Dian-chang1,SU Tian-feng1,JIANG Shi-gui1 (1. Key Lab of Aquaculture & Ecology , Quality Control of Agriculture Ministry,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China,2. Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China) |
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| Abstract: | In order to clone and analyze the differentially expressed genes of gonads of domesticated broodstock Penaeus monodon Fabricius,total RNA was extracted from the ovary and testis,and differential display technique was performed to screen the differentially expressed genes by using a set of 3 anchored primers for cDNA synthesis and 8 arbitrary primers in the PCR. The differential display DNA bands were separated by denaturing urea-polyacrylamide gel electrophoresis (PAGE) and displayed by silver staining. Fin... |
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| Keywords: | Penaeus monodon gonad differential display differentially expressed genes |
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