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一对粳稻细胞质雄性不育系与恢复系的DNA限制性片段长度的多态性分析
引用本文:朱立煌,徐吉臣,胡乃璧,李良材,杨振玉.一对粳稻细胞质雄性不育系与恢复系的DNA限制性片段长度的多态性分析[J].作物学报,1992,18(2):99-106.
作者姓名:朱立煌  徐吉臣  胡乃璧  李良材  杨振玉
作者单位:中国科学院遗传研究所; 辽宁农科院稻作所
摘    要:通过对粳稻细胞质雄性不育系秀岭 A 和 C8411的染色体限制性片段长度的多态性分析,定量地评估了它们的 F_1杂种基因组的杂合性。在评估时,总共检测了12对染色体上的122个位点,使用9种不同的限制性内切酶,结果发现,在26%的位点中有等位的差异,而且呈现多态性的位点不是随机或均匀地分布在不同的染色体上的。根据揭示多态性的限

关 键 词:杂交水稻  限制性片段长度的多态性  杂合度  
收稿时间:1992-01-05
修稿时间:1992-02-29

Restriction Fragment Length Polymorphism (RFLP) Analysis between a Cytoplasmic Male Sterile (CMS) Line and a Rescover Line of Japonica Rice
Zhu Lihuang Xu Jichen Hu Naibi Li Liangcal Yang Zhenyu.Restriction Fragment Length Polymorphism (RFLP) Analysis between a Cytoplasmic Male Sterile (CMS) Line and a Rescover Line of Japonica Rice[J].Acta Agronomica Sinica,1992,18(2):99-106.
Authors:Zhu Lihuang Xu Jichen Hu Naibi Li Liangcal Yang Zhenyu
Institution:Institute of Genetic; Academia Sinica.~*Rice Institute; Liaoning Academy of Agricultural Sciences
Abstract:Assessment of the degre of heterozygosity of the F_1 of Xiuling A(cms)/C8411(rescover)was made by RFLP analyses.Total 122 loci on the 12 rice chromosomeswere assayed with 9 different restriction enzymes in digestion of rice DNAs.Allelic dif-ferences between XiulingA and C8411 were detected at 32(26%)loci,which wereunevenly distributed on the 12 chromosomes.Two main types of RFLPs betweenXiuling A and C8411 could be distinguished according to the number of restrictionenzymes that detected polymorphism.An approach in search of correlation betweenheterosis and heterozygosity in hybrid rices is proposed for our further study.
Keywords:Hybrid rice  RFLP  Heterozygosity  
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