| 重庆稻瘟病菌不同菌株DNA指纹图谱分析 |
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| 引用本文： | 杨水英,肖崇刚,韩海波,杨静,彭洪江,何霞红.重庆稻瘟病菌不同菌株DNA指纹图谱分析[J].西南农业大学学报,2002,24(3):235-237. |
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| 作者姓名： | 杨水英 肖崇刚 韩海波 杨静 彭洪江 何霞红 |
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| 作者单位： | [1]西南农业大学植保系，重庆400716 [2]涪陵农业科学研究所，重庆涪陵408000 |
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| 基金项目： | 国家 8 63计划项目 (国科生字 1 999[1 1 3]号 ) |
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| 摘 要： | 采用rep-PCR指纹技术对70个不同来源的稻瘟病菌菌株的DNA指纹图谱进行了测定。菌株间显示了DNA图像的多态性，供试菌株分别扩增出2－15条DNA带。经聚类分析，在0．80遗传相似水平下，供试菌株分为9个遗传谱系，其中第3，6，9为优势谱系。菌株的遗传谱系与菌株的原寄主和原采集地之间均表现出较明显的相关性。
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| 关 键 词： | 重庆 稻瘟病菌 菌株 DNA 指纹图谱分析 遗传谱系 多态性 寄主 采集地 |
| 文章编号： | 1000-2642(2002)03-0235-03 |
| 修稿时间： | 2001年12月19 |
ANALYSIS ON DNA FINGERPRINTS OF DIFFERENT ISOLATES OF MAGNAPORTHE GRISEA IN CHONGQING |
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| YANG Shui-ying ,XIAO Chong-gang ,HAN Hai-bo ,YANG Jing ,PENG Hong-jiang ,HE Xia-hong.ANALYSIS ON DNA FINGERPRINTS OF DIFFERENT ISOLATES OF MAGNAPORTHE GRISEA IN CHONGQING[J].Journal of Southwest Agricultural University,2002,24(3):235-237. |
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| Authors: | YANG Shui-ying XIAO Chong-gang HAN Hai-bo YANG Jing PENG Hong-jiang HE Xia-hong |
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| Institution: | YANG Shui-ying 1,XIAO Chong-gang 1,HAN Hai-bo 2,YANG Jing 3,PENG Hong-jiang 2,HE Xia-hong 3 |
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| Abstract: | DNA fingerprints of 70 Magnaporthe grisea isolates collected from different sources in Chongqing were tested using rep-PCR technique. Polymorphism of DNA amplified fingerprints appeared among the isolates. The number of DNA amplified bands varied from 2 to 15 in them. The isolated tested were clustered into 9 genetic lineages at 0.80 genetic similarity, of which No.3, No.6 and No.9 were the dominant lineages. Obvious relation was shown to exist between the genetic lineages of the isolates, on the one hand, and their original hosts and collecting places, on the other. |
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| Keywords: | Magnaporthe grisea rep-PCR DNA fingerprint genetic lineage correlation |
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