| 蚯蚓堆制处理对不同物料中4种酶活性的影响 |
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| 引用本文： | 周颖,陈泽光,宦霞娟,薛鹏,徐伟佳,马雄,陈玉林.蚯蚓堆制处理对不同物料中4种酶活性的影响[J].西北农业学报,2009,18(6):66-69. |
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| 作者姓名： | 周颖 陈泽光 宦霞娟 薛鹏 徐伟佳 马雄 陈玉林 |
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| 作者单位： | 西北农林科技大学,动物科技学院,陕西杨凌,712100 |
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| 基金项目： | 陕西重大科技创新专项，优质高产良种奶牛选育及标准化生产技术集成与示范 |
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| 摘 要： | 旨在研究蚯蚓堆置处理作用对纤维素、半纤维素、木质素降解的影响.采用室内接种的方法,用不同C/N的玉米秸秆与纯牛粪混合物为物料饲养赤子爱胜蚓(Eisenia foetida),以成蚓与幼蚓总数和物料中纤维素酶、木聚糖酶、过氧化物酶和多酚氧化酶的酶活性为检测指标.结果表明,接种蚯蚓的试验组比未接种蚯蚓的对照组能显著增强物料中4种酶的活性;接种蚯蚓的物料C/N为25时,成蚓与幼蚓总数、纤维素酶、过氧化物酶和多酚氧化酶的酶活性较其他组高,3种酶活性随时间逐渐增强;C/N为30时,木聚糖酶活性较其他组高,并随时间逐渐减弱.蚯蚓能在堆制处理过程中加速纤维素、半纤维素和木质素的降解;当C/N=25时,蚯蚓繁殖较好,比较有利于纤维素、半纤维素、木质素降解.
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| 关 键 词： | 蚯蚓堆置处理 纤维素酶 木聚糖酶 过氧化物酶 多酚氧化酶 |
| 收稿时间： | 2009/3/9 0:00:00 |
| 修稿时间： | 2009/6/21 0:00:00 |
Four Enzyme Activities from Different Feedstocks after Treatment with Eisenia fetida |
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| ZHOU Ying,CHEN Zeguang,HUAN Xiajuan,XUE Peng,XU Weiji,MA Xiong and CHEN Yulin.Four Enzyme Activities from Different Feedstocks after Treatment with Eisenia fetida[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(6):66-69. |
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| Authors: | ZHOU Ying CHEN Zeguang HUAN Xiajuan XUE Peng XU Weiji MA Xiong and CHEN Yulin |
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| Institution: | College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, China |
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| Abstract: | To assess the role of earthworms in cellulose, hemicellulose and lignin decomposition, we carried out experiments in which the different C/N mixtures of cattle manure and corn stock were treated in laboratory with Eisenia foetida , Taking the population of earthworms and the activity of Cellulase, Xylanase, Peroxydase(POD)and Polyphenoloxidase (PPO)as indices. It was found that the presence of earthworms stimulated the activity of enzymes; Eisenia foetida showed the best reproduction and the highest activity of Cellulase, POD, and PPO at C /N = 25, significantly increasing with periods, However, Xylanase showed the highest activity at C/N = 30, decreasing with periods. Eisenia foetida can trigger the decomposition of cellulose,hemicellulose and lignin. The optimal C/N condition for the reproduction of Eisenia foetida, and the decomposition of cellulose, hemicellulose and lignin was 25. |
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| Keywords: | Vermicomposting Cellulase Xylanase Peroxydase Polyphenoloxidase |
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