| 特定电磁波照射对家兔体外受精的影响 |
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| 引用本文： | 黄依明,安民.特定电磁波照射对家兔体外受精的影响[J].福建农林大学学报(自然科学版),1994(2). |
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| 作者姓名： | 黄依明 安民 |
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| 作者单位： | 福建农业大学动物科学系，北京农业大学动物科学技术学院 |
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| 摘 要： | 家兔精液用特定电磁波（ＴＤＰ）照射３ｍｉｎ，精子体外获能采用ｍ—ＨＩＳ（１５ｍｉｎ）＋ｍ－ＤＭ（２－４ｈ）．家兔卵母细胞经ＴＤＰ照射５ｍｉｎ并体外培养１０－１２ｈ．家兔的卵泡卵母细胞经体外受精和体外培养的结果表明，经ＴＤＰ照射的精液与未经ＴＤＰ照射的卵母细胞的体外受精，原核形成率达６４．７％，对照织为４４．４％，两者差异显著（Ｐ＜０．０５），２－细胞以上的受精卵发育率分别为５６．８％和５０％（Ｐ＞０．０５），经ＴＤＰ照射的卵母细胞与未经ＴＤＰ照射的精液的体外受精，原核形成率达６３．７％，对照组为４６．７％，两者差异显著（Ｐ＜０．０５），２－细胞以上的受精卵发育率分别为５８．９％和４７．２％（Ｐ＞０．０５）、用ＴＤＰ照射精液或卵母细胞能显著提高其体外受精率．
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| 关 键 词： | 特定电磁波，体外受精，精子体外获能，家兔 |
Effects of Specific Electromagnatic Wave on in Vitro Fertilization of Rabbit |
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| Huang Yiming, An Min.Effects of Specific Electromagnatic Wave on in Vitro Fertilization of Rabbit[J].Journal of Fujian Agricultural and Forestry University,1994(2). |
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| Authors: | Huang Yiming An Min |
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| Abstract: | Group one: rabbit semen was radiated with Specific Electromagnetic Wave (TDP) for 3 minutes and sperm capacitation was carried out with m-HIS for 15 minutes, and in m-DM for 2-4 hours, then fertilited with normal oocytes in vitro. Group two: normal oocytes were radiated with TDP for 5 minutes and cultivated for 10-12 hours, and then fertilited with normal sperm in vitro. The results showed that the rate of pronucleus formation was 64.7% in treated group and 44.4% in the control (P< 0.05) and the rate of embryo development was 56.8% and 50.0% respectively (P>0.05) in group one. The rate of pronucleus formation was 63.7% in treated group and 46.7% in the control (P<0.05), the rate of embryo development was 58.9%and 47.2% respectively (P>0.05). The results indicated that fertilitation rate in rabbit was significantly incresed by the TDP radiation to semen or oocytes. |
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| Keywords: | specific electromagnetic wave in vitro fertilization in vitro capacitation of sperm rabbit |
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