| 一株猪流行性腹泻病毒S、M、N基因的遗传变异分析 |
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| 引用本文： | 易新健,刘准,张翠翠,臧传佼,徐健,陈申秒.一株猪流行性腹泻病毒S、M、N基因的遗传变异分析[J].动物医学进展,2017,38(2). |
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| 作者姓名： | 易新健 刘准 张翠翠 臧传佼 徐健 陈申秒 |
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| 作者单位： | 山东滨州沃华生物工程有限公司,山东省动物病原微生物工程实验室,山东滨州 256600 |
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| 摘 要： | 为研究猪流行性腹泻病毒(PEDV)CV777疫苗毒株与流行毒株的遗传变异和抗原位点的差异性,以RT-PCR进行PEDV CV777疫苗株和JS2016流行株S、M、N 3个基因的克隆测序,进行PEDV S、M、N 3个基因的核酸序列同源性分析,并通过软件比对CV777与JS2016毒株在这3个基因上的抗原差异。S、M、N基因的同源性分析表明流行毒株与CV777存在变异,但同源性在93%以上;免疫原性预测结果显示2个毒株在S、M、N 3个基因上的抗原区域存在较小的差异。
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| 关 键 词： | 猪流行性腹泻病毒 CV777 S基因 M基因 N基因 |
Genetic Correlation and Immunogenicity Analysis of S,M,N Genes ofPorcine Epidemic Diarrhea Virus |
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| YI Xin-jian,LIU Zhun,ZHANG Cui-cui,ZANG Chuan-jiao,XU Jian,CHEN Shen-miao.Genetic Correlation and Immunogenicity Analysis of S,M,N Genes ofPorcine Epidemic Diarrhea Virus[J].Progress In Veterinary Medicine,2017,38(2). |
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| Authors: | YI Xin-jian LIU Zhun ZHANG Cui-cui ZANG Chuan-jiao XU Jian CHEN Shen-miao |
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| Abstract: | To carry out the genetic variation and diversity of antigenic sites between CV777 vaccine strain and epidemic strain of porcine epidemic diarrhoea virus,RT-PCR was used to clone the S,M and N genes of PEDV CV777 vaccine strain and JS2016 strain,then the PCR products were sequenced for analyzing the nucleotide sequences and antigenic sites.The nucleotide sequence of CV777 vaccine strain had some variation compared to JS2016 strain,but the homology was higher than 93%.The prediction result of immunogenicity showed that less differences of antigenic sites of S,M and N genes exist between CV777 vaccine strain and JS2016 strain. |
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| Keywords: | Porcine epidemic diarrhoea virus CV777 S gene M gene N gene |
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