| 福建省羊传染性脓疱病毒F1L、B2L和VIR基因的遗传变异分析 |
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| 引用本文： | 林裕胜,江锦秀,江斌,游伟,胡奇林.福建省羊传染性脓疱病毒F1L、B2L和VIR基因的遗传变异分析[J].动物医学进展,2017,38(2). |
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| 作者姓名： | 林裕胜 江锦秀 江斌 游伟 胡奇林 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所,福建福州,350013 |
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| 基金项目： | 福建省科技计划项目，福建省公益性科研专项 |
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| 摘 要： | 为探明2014年-2015年在福建省流行的羊传染性脓疱病毒(ORFV)遗传变异情况,对10株ORFV流行毒株的F1L、B2L和VIR基因进行克隆、测序及分析。结果表明,10株ORFV F1L基因之间的核苷酸序列同源性为97.6%~100%,与国内株的核苷酸序列同源性为96.8%~99.7%,与NZ2参考株的核苷酸序列同源性为96.3%~97.1%;同NZ2参考株比较,FJ-YT2014缺失2个氨基酸;10株ORFV B2L基因之间核苷酸序列同源性为97.5%~99.9%,与国内株的核苷酸序列同源性为96.7%~99.5%,与NZ2参考株的核苷酸序列同源性为96.7%~97.7%;10株ORFV VIR基因之间的核苷酸序列同源性为95.8%~99.5%,与国内株的核苷酸序列同源性为94.6%~99.6%,与NZ2参考株的核苷酸序列同源性为94.6%~96.4%。基于基因核苷酸序列的遗传进化分析表明,10株ORFV F1L基因与福建省分离株、山西株和新疆株亲缘关系较近;10株ORFV B2L基因与新疆、山西、德国毒株亲缘关系较近;10株ORFV VIR基因与台湾、新疆株亲缘关系较近。结果提示,当前福建省流行的ORFV F1L、B2L和VIR基因尚未出现明显变异,但是其F1L、B2L和VIR基因核苷酸序列之间普遍存在异质性。
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| 关 键 词： | 羊传染性脓疱病毒 F1L基因 B2L基因 VIR基因 变异分析 |
Genetic Variation Analysis of F1L,B2L and VIR Genes of Orf Virus in Fujian Province |
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| LIN Yu-sheng,JIANG Jin-xiu,JIANG Bin,YOU Wei,HU Qi-lin.Genetic Variation Analysis of F1L,B2L and VIR Genes of Orf Virus in Fujian Province[J].Progress In Veterinary Medicine,2017,38(2). |
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| Authors: | LIN Yu-sheng JIANG Jin-xiu JIANG Bin YOU Wei HU Qi-lin |
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| Abstract: | To investigate the variation in F1L,B2L and VIR genes of Orf virus(ORFV),a total of 10 ORFV F1L,B2L and VIR genes were cloned and sequenced from different farms in Fujian province during 2014-2015.The results showed that the nucleotide sequences of F1L gene shared 97.6%-100% similarities within them,shared 96.8%-99.7%similarities with domestic strains and shared 96.3%-97.1% similarities with NZ2 strain.Compared with NZ2 strain,there were 2 amino acids deletion.The nucleotide sequence of B2L gene shared 97.5%-99.9% similarities within them,shared 96.7%-99.5% similarities with domestic strains and shared 96.7%-97.7%similarities with NZ2 strain.The nucleotide sequence of VIR gene shared 95.8%-99.5% similarities with them,shared 94.6%-99.6%similarities with domestic strains and shared 94.6%-96.4% similarities with NZ2 strain.Phylogenetic analysis showed that F1L gene of the 10 ORFV strains had a closer relationship with the Fujian,Shanxi and Xinjiang strains;B2L gene of the 10 ORFV strains had a closer relationship with the Xinjiang,Shanxi and Germany strains;VIR gene of 10 ORFV strains had a closer relationship with the Taiwan and Xinjiang strains.The results indicated that 10 ORFV F1L,B2L and VIR genes have not a significant variation,but 10 ORFV F1L,B2L and VIR genes have widespread heterogeneity between nucleotide sequences. |
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| Keywords: | Orf virus F1L gene B2L gene VIR gene variation analysis |
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