| 羊布鲁菌快速检测技术应用研究 |
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| 引用本文： | 葛婷,雷程红,王振宝,卞赛赛,樊新丽.羊布鲁菌快速检测技术应用研究[J].动物医学进展,2017,38(1). |
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| 作者姓名： | 葛婷 雷程红 王振宝 卞赛赛 樊新丽 |
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| 作者单位： | 1. 新疆农业大学动物医学学院,新疆乌鲁木齐,830052;2. 伊犁出入境检验检疫局,新疆伊宁,835000 |
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| 基金项目： | 新疆农业大学研究生产学研项目，国家级大学生创新项目 |
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| 摘 要： | 通过用4种方法对羊布鲁菌的检测,确立适用于基层应用的羊布鲁菌快速检测技术。http://www.mdxycn.com/采用浊度仪LAMP、金属浴LAMP、荧光目视检测试剂盒LAMP和普通PCR共4种方法对羊血液中的布鲁菌进行检测,比较其检测结果。结果表明,共检测162份羊血液,布鲁菌检出率分别为浊度仪LAMP为8.6%、金属浴LAMP为8.6%、荧光目视检测试剂盒为8.6%、普通PCR为6.2%。LAMP较普通PCR方法特异性强且灵敏度高;金属浴LAMP方法操作简便,无需特殊设备,更适用于基层应用。
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| 关 键 词： | 羊 布鲁菌 环介导等温扩增技术 检测 |
Study on Applification of Rapid Detection Technology of Brucella in Sheep |
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| GE Ting,LEI Cheng-hong,WANG Zhen-bao,BIAN Sai-sai,FAN Xin-li.Study on Applification of Rapid Detection Technology of Brucella in Sheep[J].Progress In Veterinary Medicine,2017,38(1). |
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| Authors: | GE Ting LEI Cheng-hong WANG Zhen-bao BIAN Sai-sai FAN Xin-li |
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| Abstract: | The study used four detection methods aimed to confirm methods for sheep Brucella rapid detection and applied to grass-roots unit in Yili,Xinjiang.Brucella in sheep blood were detected using Turbidity LAMP,Metal bath LAMP,Fluorescent Detection Reagent LAMP and PCR method.Then the four detection results were compared.162 sheep blood samples were used to detect Brucella,and the respective detection rates were 8.6%,8.6%,8.6%,6.2%.According to the results,LAMP were better than PCR method on sensitivity and specificity.Metal bath LAMP method was easy to operate without special equipment,and it is more applicable to grass-roots unit. |
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| Keywords: | sheep Brucella loop-mediated isothermal nucleic acid technique detection |
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