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| 我国五省区部分猪场高致病性猪蓝耳病毒感染情况检测与分析 | |
| 引用本文： | 高许雷,吴发兴,朱紫祥,李平,张燕霞,张志,刘爽,李晓成,黄保续.我国五省区部分猪场高致病性猪蓝耳病毒感染情况检测与分析[J].动物医学进展,2009,30(11):32-36. |
| 作者姓名： | 高许雷 吴发兴 朱紫祥 李平 张燕霞 张志 刘爽 李晓成 黄保续 |
| 作者单位： | 1. 青岛农业大学,山东青岛,266109;中国动物卫生与流行病学中心,山东青岛,266032 2. 中国动物卫生与流行病学中心,山东青岛,266032 3. 中国动物卫生与流行病学中心,山东青岛,266032;西北农林科技大学,陕西杨陵,712100 4. 中国动物卫生与流行病学中心,山东青岛,266032;新疆农业大学,新疆乌鲁木齐,830052 |
| 基金项目： | 科技部"十一五"科技支撑计划项目 |
| 摘 要： | 对2008年-2009年我国五省区发病猪场235份、屠宰场的218份样品进行了高致病性猪蓝耳病病毒(HP-PRRSV)RT-PCR检测,挑选具有代表性的HP-PRRSV阳性样品进行HP-PRRSV ORF5基因扩增、测序及分析.结果表明,这五个省区发病场蓝耳病的检出率平均74.6%.屠宰场的检出率平均44%,混合感染主要以二重感染为主,且发病场较屠宰场严重.对获得的13株HP-PRRSV ORF5进行测序分析,发现序列长度均为603 bp,未见缺失或插入,仅在9 aa～29 aa存在点突变;序列比较发现,与普通株标准序列VR-2332株核苷酸同源性达85.9%～87.2%;与高致病性毒株的同源性JXA1-06核苷酸同源性达96.0%～99.0%.而其推导氨基酸序列与普通型、高致病性毒株的同源性分别为87.1%～88.1%和97.5%～98.0%.从遗传进化以及变异情况看,获得的PRRSV ORF5序列均为与JXA1类似的高致病性PRRSV序列,与JXA1和HB-1同属美洲型中的一个亚群. |
| 关 键 词： | 高致病性猪蓝耳病病毒 RT-PCR检测 ORF5基因 |
Detection and Analysis on Infection Situation of High Pathogenic Porcine reproductive and respiratory syndrome viruse in Five Provinces of China |
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| GAO Xu-lei,WU Fa-xing,ZHU Zi-xiang,LI Ping,ZHANG Yan-xia,ZHANG Zhi,LIU Shuang,LI Xiao-cheng,HUANG Bao-xu.Detection and Analysis on Infection Situation of High Pathogenic Porcine reproductive and respiratory syndrome viruse in Five Provinces of China[J].Progress In Veterinary Medicine,2009,30(11):32-36. | |
| Authors: | GAO Xu-lei WU Fa-xing ZHU Zi-xiang LI Ping ZHANG Yan-xia ZHANG Zhi LIU Shuang LI Xiao-cheng HUANG Bao-xu |
| Institution: | GAO Xu-lei1,2,WU Fa-xing2,ZHU Zi-xiang2,3,LI Ping2,4,ZHANG Yan-xia2,ZHANG Zhi2,LIU Shuang2,LI Xiao-cheng2,HUANG Bao-xu1,2(1.Qingdao Agriculture University,Qingdao,Shandong,266109,China,2.China Animal Health And Epidemiology Center,266032,3.Northwest A&F University,Yangling,Shaanxi,712100,4.Xinjiang Agriculture University,Urmuqi,Xinjiang,830052,China) |
| Abstract: | During 2008 to 2009,235 samples were sampled from some pig farms that existed PRRS and 218 samples were sampled from some slaughterhouses,respectively,from 5 provinces in China.All these samples were tested to detect PRRSV by RT-PCR.After the detection,some typical HP-PRRSV positive samples were chosen to determine the ORF5 genes.The research showed that PRRSV infection rate in pig farms were 74.6%,and 44% in slaughterhouses.Polyinfection also existed,which mainly were double infection,the infection rate in... |
| Keywords: | HP-PRRSV RT-PCR detection ORF5 gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |