Induced resistance to<Emphasis Type="Italic">Cladosporium cucumerinum</Emphasis> in cucumber by pectinases extracted from<Emphasis Type="Italic">Penicillium oxalicum</Emphasis> |
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| Authors: | Xiawei?Peng Email author" target="_blank">Hongxun?ZhangEmail author Zhihui?Bai Baojv?Li |
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| Institution: | (1) Dept. of Environmental Biotechnology, Research Center for Eco-Environmental Sciences and Graduate School, Chinese Academy of Sciences, 100085 Beijing, China;(2) Inst. of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081 Beijing, China |
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| Abstract: | Pectinases extract (PE) from the fermentation product ofPenicillium oxalicum BZH-2002 was tested for its ability to induce protection against scab caused byCladosporium cucumerinum on cucumber (Cucumis sativus L.) plants. Seedlings with one true leaf were sprayed with various concentrations of PE (10–200 units ml−1) 3 days before inoculation withC. cucumerinum. Results showed that the induced local protection against the pathogen was dose-dependent when the concentrations of PE were
between 20 and 120 units ml−1; systemically induced resistance against the pathogen was not observed. Boiled PE had a slight effect on disease reduction.
Commercial pectinases prepared fromAspergillus niger showed lower protection against scab compared with PE when they were used at the same concentration of enzyme activity. No
inhibitory activity was observed on conidial germination or germ-tube growth ofC. cucumerinum. PE was further evaluated for its enhancement of defense-related enzymes. Peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine
ammonia lyase (PAL) increased in cucumber seedlings after treatment with PE. PO and PPO remained at a higher level in PE-pretreated
seedlings throughout the experiment period whether pathogen-inoculated or non-inoculated, whereas PAL activity began to decrease
2 days after PE treatment.
http://www.phytoparasitica.org posting July 14, 2004. |
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| Keywords: | Cucumber scab induced protection pectinase pectinase extract Penicillium oxalicum |
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