| 仙客来热激蛋白基因HSP21.4植物表达载体构建及鉴定 |
| |
| 引用本文： | 车野,李凤兰,闻静,王莹,胡宝忠.仙客来热激蛋白基因HSP21.4植物表达载体构建及鉴定[J].东北农业大学学报,2008,39(8). |
| |
| 作者姓名： | 车野 李凤兰 闻静 王莹 胡宝忠 |
| |
| 作者单位： | 东北农业大学生命科学院,哈尔滨,150030 |
| |
| 摘 要： | 试验以仙客来热激蛋白基因HSP21.4为材料,经PCR扩增的方法在目的片断两端添加SacⅠ位点,然后与植物表达载体pBI121连接,转化感受态大肠杆菌,经PCR和SacⅠ及XbaⅠ酶切验证,确定已将该热激蛋白基因连接到植物表达载体上,将重组质粒命名为pBI-CpHSP21.4,并采用冻融法完成了对pBI-CpHSP21.4质粒的农杆菌转化,为验证该热激蛋白基因的功能及转基因植物表达奠定基础。
|
| 关 键 词： | 热激蛋白 植物表达载体 载体构建 |
Construction and identification of plant expression vectors containing HSP21.4 gene in Cyclamen |
| |
| CHE Ye,LI Fenglan,WEN Jing,WANG Ying,HU Baozhong.Construction and identification of plant expression vectors containing HSP21.4 gene in Cyclamen[J].Journal of Northeast Agricultural University,2008,39(8). |
| |
| Authors: | CHE Ye LI Fenglan WEN Jing WANG Ying HU Baozhong |
| |
| Abstract: | The experiment was conducted with HSP21.4 gene.Adding Sac I site to the endpoints of objective genes by using PCR amplification,then ligated with pBI121.The recombinant plasmid was transferred into competent cells of Escherishia coli DH5α.The PCR and Sac I digestion test showed that the HSP21.4 gene was constructed into plant expression vector,and recombinant plasmid designated as pBI-CpHSP21.4.The recombinant plasmid was transferred into competent cells of Agrohacterium tumefaciens LBA4404.One A.tumefaciens clone harboring recombinant plasmind pBI-CpHSP21.4 was obtained.It lays a foundation for testing function of HSP21.4 gene and transgenic plant expressing. |
| |
| Keywords: | heat-shock protein plant expression vector vector construction |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
