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Expression of connexin 26 and bovine papillomavirus E5 in cutaneous fibropapillomas of cattle |
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| Authors: | Maria Angelica Silva Gennaro Altamura Annunziata Corteggio Franco Roperto Florentina Bocaneti Elena Velescu Antonio C Freitas Cybelle CR Carvalho Karen PS Cavalcanti Giuseppe Borzacchiello |
| Institution: | 1. Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rêgo, 1235, Cidade Universitária, 50740521 Recife, Pernambuco, Brazil;2. Department of Pathology and Animal Health, University of Naples Federico II, Via Veterinaria 1, 80137 Napoli, Italy;3. Department of Public Health, Faculty of Veterinary Medicine, University of Agriculture Sciences and Veterinary Medicine Ion Ionescu de la Brad, Iasi, Romania;1. Charité-Universitätsmedizin Berlin, Department of Anesthesiology, CCM, Charitéplatz 1, 10117 Berlin, Germany;2. Freie Universität Berlin, Department of Veterinary Medicine, Clinic for Ruminants and Swine, Königsweg 65, 14163 Berlin, Germany |
| Abstract: | Bovine papillomaviruses (BPVs) can infect epithelial cells and fibroblasts, inducing fibropapillomas in cattle. Gap junctions are communication channels between cells composed of connexins (Cxs). This study evaluated expression of Cx26 and the major BPV oncoprotein E5 in bovine cutaneous fibropapillomas. BPV DNA was amplified from 20/20 fibropapillomas and 3/3 samples of normal skin. All fibropapillomas (20/20) were positive by immunostaining for E5, whereas the three normal skin samples were negative. Cx26 was expressed faintly in the normal skin epithelium. Positive cytoplasmic and juxtanuclear immunoreactivity for Cx26 was evident in 18/20 (90%) fibropapillomas. Western blot analysis demonstrated higher expression of Cx26 in 6/6 fibropapillomas compared to normal skin samples. |
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