Identification of a novel gill-specific calpain from rainbow trout (Oncorhynchus mykiss) |
| |
| Authors: | M Salem C E Rexroad J Yao |
| |
| Institution: | (1) Division of Animal and Veterinary Sciences, West Virginia University, Morgantown, WV 26506, USA;(2) National Center for Cool and Cold Water Aquaculture, Kearneysville, WV 25430, USA |
| |
| Abstract: | Calpains are calcium-dependent neutral proteases responsible for many cellular functions. The two forms of calpain ubiquitously
expressed in mammalian tissues are known as μ-calpain and m-calpain. We report here the identification of a novel calpain
that is similar to but distinct from the μ- and m-calpains in rainbow trout. The cDNA of the novel gene is 2623 bp in length
with a single open reading frame. The predicted protein (676 amino acids) contains the conserved calpain characteristic domains
that include: domain I (pro peptide), II (cysteine catalytic site), III (electrostatic switch), and IV (calmodulin-like) with
five Ca2+-binding EF hands. Northern blot and RT-PCR analyses demonstrated that the novel calpain gene is predominantly expressed in
rainbow trout gills. Comparison of the novel protein with the ubiquitously expressed calpains and several mammalian tissue-specific
calpains revealed that the novel calpain is an orthologue of the mammalian digestive tract specific calpain (calpain 9). |
| |
| Keywords: | calpain gill rainbow trout |
| 本文献已被 SpringerLink 等数据库收录！ |
|
