| 小白菜耐盐变异体的诱导及离体筛选 |
| |
| 引用本文： | 王亦菲,黄剑华,陆瑞菊,陈志伟,何婷,周润梅.小白菜耐盐变异体的诱导及离体筛选[J].核农学报,2009,23(6):951-957. |
| |
| 作者姓名： | 王亦菲 黄剑华 陆瑞菊 陈志伟 何婷 周润梅 |
| |
| 作者单位： | 上海市农业科学院生物技术研究所,上海市农业遗传育种重点实验室,上海,201106 |
| |
| 基金项目： | 上海市科委重大攻关项目 |
| |
| 摘 要： | 以3份优良小白菜品种为供试材料，研究了培养基中不同NaCl浓度对种子萌发和茎端组织存活及生长的影响；不同平阳霉素浓度及时间的处理对种子萌发和茎端组织存活及生长的影响。结果表明：供试种子的萌发率和茎端组织的存活率随培养基中NaCl浓度的上升急剧下降，平阳霉素处理对茎端组织存活率的抑制明显大于对种子萌发率的影响，平阳霉素处理后存活的种子和茎端组织经NaCl胁迫筛选，筛选出耐NaCl胁迫力明显提高的存活苗，存活苗经无性繁殖，可以保持较高的耐NaCl能力。
|
| 关 键 词： | 小白菜 NaCl胁迫 平阳霉素 种子萌发 茎端组织 耐盐变异体 |
| 收稿时间： | 2009-07-15 |
Induction and in vitro Selection of Variants with Salt tolerance from Brassica. chinensis |
| |
| WANG Yi-fei,HUANG Jian-hua,LU Rui-ju,CHEN Zhi-wei,HE Ting,ZHOU Run-mei.Induction and in vitro Selection of Variants with Salt tolerance from Brassica. chinensis[J].Acta Agriculturae Nucleatae Sinica,2009,23(6):951-957. |
| |
| Authors: | WANG Yi-fei HUANG Jian-hua LU Rui-ju CHEN Zhi-wei HE Ting ZHOU Run-mei |
| |
| Institution: | Biotech Research Institute, Shanghai Academy of Agricultural Sciences; Shanghai Key Lab of Agricultural Genetics and Breeding, Shanghai 201106 |
| |
| Abstract: | Using 3 Brassica chinensis cultivars as donor materials, the effects of NaCl stress on seed-germination and stem apexes growth of the materials and the effects of Pingyangmycin (PYM) concentrations and treating time on seed-germination and survival rate of stem apexes were investigated under tissue culture condition. The results showed that the seed germination and stem apexes growth of the materials was decreased with the increase of NaCl concentration and PYM concentration and treating time. The inhibition of the PYM treatment for surival of stem apexes was higher than that of seed germination. The survival plantests with enhanced NaCl-tolerance were obtained by the PYM treatment and NaCl-stress selection. The enhanced NaCl-tolerance of the plantlets could be maintained by propagation. |
| |
| Keywords: | Brassica chinensis NaCl stress pingyangmycin(PYM) seed germination stem apexes salt tolerant variant |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《核农学报》浏览原始摘要信息 |
| 点击此处可从《核农学报》下载免费的PDF全文 |
