| HPLC法测定穿心莲中穿心莲内酯和脱水穿心莲内酯的含量 |
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| 引用本文： | 颜军,苟小军,邬晓勇,何钢,雍林.HPLC法测定穿心莲中穿心莲内酯和脱水穿心莲内酯的含量[J].安徽农业科学,2009,37(8):3576-3577. |
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| 作者姓名： | 颜军 苟小军 邬晓勇 何钢 雍林 |
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| 作者单位： | 成都大学中药化学实验室,四川成都,610106 |
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| 摘 要： | 目的]建立对穿心莲中穿心连内酯和脱水穿心莲内酯进行同时测定的HPLC法。方法]采用色谱柱Alltech C18（4．6mm×250mm，5μm）；以甲醇-水（54：46）为流动相；流速1．0mL／min；检测波长225nm；柱温35℃。结果]穿心莲内酯及脱水穿心莲内酯的线性范围分别为0．0338～2．16μg（r=0．9992）和0．0325～2．08μg（r=0．9996），平均回收率分别为98．7％、96．5％。结论]该方法准确、简便、灵敏度高、重复性好，适用于穿心莲中穿心莲内酯和脱水穿心莲内酯的同时测定。
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| 关 键 词： | HPLC 穿心莲 穿心莲内酯 脱水穿心莲内酯 |
Determination of Andrographolide and Dehydroandrographolide in Andrographis paniculata Nees by HPLC |
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| YAN Jun et al.Determination of Andrographolide and Dehydroandrographolide in Andrographis paniculata Nees by HPLC[J].Journal of Anhui Agricultural Sciences,2009,37(8):3576-3577. |
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| Authors: | YAN Jun |
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| Institution: | YAN Jun et al(The Laboratory of Traditional Chinese Medicine Chengdu University,Chengdu,Sichuan 610106) |
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| Abstract: | Objective ] The aim was to determine andrographolide and dehydroandrographolide in Andrographis paniculata Nees by HPLC method. Method]The HPLC method was performed with a Alhech C18 column（4.6 mm × 250 mm,5 μm） at 35 ℃ , and the mobile phase methanol-water（54：46,v/v） mixture, the flow rate 1.0 ml/min, the detection wavelength 225 nm. Result ] The andrographolide and dehydroandrographolide linear range Were 0. 033 8 -2.16 μg （r = 0. 999 2 ） and 0. 032 5 -2.08 μg（ r = O. 999 6） respectively and the average recovery rates were 98.7% and 96.5%. Conclusion ] The method was simple, sensitive, repetitive and suitable for simultaneous determination of rographolide and dehydroandrographolide. |
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| Keywords: | HPLC Andrographis paniculata Nees Andrographolide Dehydroandrographolide |
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