| 鹿茸干细胞内[Na+]测定及其影响因素鉴定 |
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| 引用本文： | 赵海平,陈广信,孙红梅,褚文辉,张伟,李春义.鹿茸干细胞内[Na+]测定及其影响因素鉴定[J].中国农业大学学报,2019,24(3):78-85. |
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| 作者姓名： | 赵海平 陈广信 孙红梅 褚文辉 张伟 李春义 |
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| 作者单位： | 中国农业科学院特产研究所/特种经济动物分子生物学国家重点实验室/吉林省鹿茸工程研究中心;长春科技学院 |
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| 基金项目： | 吉林省重点科技攻关项目（20150204071NY） |
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| 摘 要： | 为探索鹿茸的形态发生与鹿茸干细胞(包括发生干细胞和再生干细胞)内Na~+]的关系有关,以鹿茸干细胞为模型,研究不同干性的哺乳动物干细胞内Na~+]的差异,并鉴定引起这种差异的电压门控性钠离子通道(NaV)因素。采集并培养具有不同干性的梅花鹿鹿茸干细胞,包括生茸区骨膜细胞(AP细胞,鹿茸发生干细胞)和角柄骨膜细胞(PP细胞,鹿茸再生干细胞,其干性低于AP细胞),以鹿普通脸部骨膜细胞(FP细胞)作为对照,并采用CoroNa染色方法,通过荧光强度来分析不同类型细胞内Na~+]的差异,结合PCR方法鉴定转录水平上NaV基因的差异,并分别用睾酮和MS-222对细胞进行处理,观测其对细胞内Na~+]的影响。结果表明:鹿茸干细胞内Na~+]高于FP细胞,其中AP细胞内Na~+]高于PP细胞;NaV1.1基因在AP细胞中特异性转录;睾酮对这3种细胞内Na~+]水平没有显著影响;但是,MS-222处理能够在一定程度上降低细胞内Na~+]。本研究发现:鹿茸干细胞内Na~+]与其干性一致,干性高的AP细胞内Na~+]也相对较高;NaV1.1基因在转录水平上的差异,可能是造成AP细胞内Na~+]高的主要原因;干扰NaV的MS-222能够在一定程度上降低细胞内Na~+]。说明哺乳动物器官的发生和再生可能与低等动物器官上的发现类似,都与细胞内Na~+]有关。
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| 关 键 词： | 鹿茸干细胞 发生和再生 细胞内钠离子浓度 电压门控性钠离子通道 |
| 收稿时间： | 2018/6/11 0:00:00 |
Measurement of the intracellular [Na+] of antler stem cells and identification of regulation factors |
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| ZHAO Haiping,CHEN Guangxin,SUN Hongmei,CHU Wenhui,ZHANG Wei and Li Chunyi.Measurement of the intracellular [Na+] of antler stem cells and identification of regulation factors[J].Journal of China Agricultural University,2019,24(3):78-85. |
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| Authors: | ZHAO Haiping CHEN Guangxin SUN Hongmei CHU Wenhui ZHANG Wei and Li Chunyi |
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| Institution: | Institute of Special Animal and Plant Sciences/State Key Laboratory for Molecular Biology of Special Economic Animals/Deer Antler Engineering Research Center, Chinese Academy of Agricultural Sciences, Changchun 130112, China,Institute of Special Animal and Plant Sciences/State Key Laboratory for Molecular Biology of Special Economic Animals/Deer Antler Engineering Research Center, Chinese Academy of Agricultural Sciences, Changchun 130112, China,Institute of Special Animal and Plant Sciences/State Key Laboratory for Molecular Biology of Special Economic Animals/Deer Antler Engineering Research Center, Chinese Academy of Agricultural Sciences, Changchun 130112, China,Institute of Special Animal and Plant Sciences/State Key Laboratory for Molecular Biology of Special Economic Animals/Deer Antler Engineering Research Center, Chinese Academy of Agricultural Sciences, Changchun 130112, China,Institute of Special Animal and Plant Sciences/State Key Laboratory for Molecular Biology of Special Economic Animals/Deer Antler Engineering Research Center, Chinese Academy of Agricultural Sciences, Changchun 130112, China and Institute of Special Animal and Plant Sciences/State Key Laboratory for Molecular Biology of Special Economic Animals/Deer Antler Engineering Research Center, Chinese Academy of Agricultural Sciences, Changchun 130112, China;Changchun Sci-Tech University, Changchun 130600, China |
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| Abstract: | |
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| Keywords: | antler stem cells generation and regeneration intracellular [Na+] voltage-gated sodium channel |
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