| 甘肃产柴胡柴胡皂苷a含量测定及抗抑郁作用评价 |
| |
| 引用本文： | 宁艳梅,杨秀娟,杨韬.甘肃产柴胡柴胡皂苷a含量测定及抗抑郁作用评价[J].中兽医医药杂志,2014(6):34-37. |
| |
| 作者姓名： | 宁艳梅 杨秀娟 杨韬 |
| |
| 作者单位： | 甘肃中医学院药学院,甘肃兰州730000 |
| |
| 基金项目： | 甘肃中医学院中青年科研基金项目（编号：BH2012-024） |
| |
| 摘 要： | 目的：比较甘肃产柴胡不同炮制品中柴胡皂苷a的含量及其对小鼠的抗抑郁作用。方法：采用HPLC测定甘肃产柴胡不同炮制品中柴胡皂苷a含量,并通过自主活动、悬尾和强迫游泳实验及脑内5-HT及NE含量的测定,比较柴胡不同炮制品对行为绝望抑郁模型小鼠的影响。结果：甘肃产柴胡不同炮制品中柴胡皂苷a的含量以酒柴胡含量最高,鳖血柴胡次之。不同炮制品均能缩短两种行为绝望模型小鼠的不动时间,以鳖血柴胡及醋柴胡效果最明显;不同炮制品均可提高小鼠脑内5-HT含量,以鳖血柴胡效果最显著,具有显著性差异。结论：炮制可使甘肃产柴胡皂苷a含量发生变化,并改善小鼠的绝望行为,增加脑内5-HT含量而显示抗抑郁作用,有良好的开发前景。
|
| 关 键 词： | 甘肃产柴胡 炮制品 柴胡皂苷a 行为绝望抑郁模型 小鼠 |
Determination of Saikosaponin-a in Radix bupleuri in Gansu Province and appraisement of its antidepressant activity |
| |
| NING Yan-mei,YANG Xiu-juan,YANG Tao.Determination of Saikosaponin-a in Radix bupleuri in Gansu Province and appraisement of its antidepressant activity[J].Journal of Traditional Chinese Veterinary Medicine,2014(6):34-37. |
| |
| Authors: | NING Yan-mei YANG Xiu-juan YANG Tao |
| |
| Institution: | (College of Pharmacy, Gansn University of Traditional Chinese Medicine, Lanzhou Gansu 730000. China) |
| |
| Abstract: | Objective： To determine saikosaponin-a in different processed products of Radix bupleuri in Gansu Province and study its antidepressant effect. Methods： HPLC was used to test the content of saikosaponin-a in different processed products of Radix bupleuri and the antidepressant effects were observed by using tile spontaneous activities test, tail suspension test. forced swimming test in mice, and 5-HT and NE in brain were detected and to be compared. Results： The content of saikosaponin-a in wine processing Radix bupleuri was the highest, then was soli-shelled turtle blood-baked ones. Different processed products could all reduce the immobility time of mice in two kinds of behavioral despair model, especially soft-shelled turtle bloodbaked ones and vinegar-baked ones. Different processed products could all increase the 5-HT level in mice brain, especially soft-shelled turtle blood-baked ones. Conclusion： Radix bupleuri in Gansu Province has promising developmenl prospect. Processing can change its saikosaponin-a content and enhance its antidepressant effect by improving behavioral despair of mice and increasing the 5-HT level in brain. |
| |
| Keywords: | Radix bupleuri in Gansu Province processed products saikosaponin-a behavioral despair animal models： mice |
| 本文献已被 维普 等数据库收录！ |
|
