|
|||||
|
|
| 鹅细小病毒NS1基因植物真核表达载体的构建 | |
| 引用本文： | 李艳梅,张廷荣,吴春涛,沈涛,朱新产.鹅细小病毒NS1基因植物真核表达载体的构建[J].西北农业学报,2006,15(4):96-99. |
| 作者姓名： | 李艳梅 张廷荣 吴春涛 沈涛 朱新产 |
| 作者单位： | 1. 莱阳农学院动物科技学院,山东青岛,266109 2. 山东农业大学动物科技学院,山东泰安,271018 3. 山东信得药业有限公司,山东青岛,266061 4. 莱阳农学院生命科学学院,山东青岛,266109 |
| 基金项目： | 山东省青岛市科技发展基金 |
| 摘 要： | 根据发表的鹅细小病毒GPV B株全基因组设计一对引物,采用PCR方法扩增出鹅细小病毒扬州株(GPV YZ)非结构蛋白基因NS1,克隆到植物表达载体pBI121中,转化至大肠杆菌感受态细胞JM109。经菌液PCR、双酶切及质粒PCR鉴定后,将阳性重组质粒转化感受态农杆菌LBA4404。经菌液PCR及质粒PCR分析,获得了真核表达载体pBI121-NS1,该载体的构建为进一步研究鹅细小病毒NS1基因的分子生物学特性打下良好基础。 |
| 关 键 词： | 鹅细小病毒 NS1基因 农杆菌LBA4404 真核植物表达载体 |
| 文章编号： | 1004-1389（2006）04-0096-04 |
| 收稿时间： | 2005-12-19 |
| 修稿时间： | 2006-01-20 |
Production of Vegetable Eukaryotic Expressing Vector Containing NS1 of Goose Parvovirus |
|
| LI Yan-mei,ZHANG Ting-rong,WU Chun-tao,SHEN Tao and ZHU Xin-chan.Production of Vegetable Eukaryotic Expressing Vector Containing NS1 of Goose Parvovirus[J].Acta Agriculturae Boreali-occidentalis Sinica,2006,15(4):96-99. | |
| Authors: | LI Yan-mei ZHANG Ting-rong WU Chun-tao SHEN Tao and ZHU Xin-chan |
| Institution: | 1. Department of Animal Sci-Tech, Laiyang Agricultural College, Qingdao Shandong 266109, China;2. College of Animal Sci-Tech Shandong Agricultural University, Taian Shandong 271018, China;3. Shandong Sinder Pharmaceuticals Company, Qingdao Shandong 266061,Chinas;4. Department of Life Science, Laiyang Agricultural College, Qingdao Shandong 266109, China |
| Abstract: | A pair of primers was designed according to the sequences published by GenBank in order to amplify NS1 gene of goose parvovirus Yangzhou strains(GPV YZ).The NS1 gene was obtained by PCR,and cloned into the pBI121 vector,and transformed into the E.coli component JM109.The expressing vector was identified by PCR and enzyme digestion and then transformed into agrobacterium tumefaciens LBA4404.After the identification by PCR from LBA4404 and pBI121-NS1 vector,the specific expression vector of GPV NS1 was constructed successfully.The construction of recombinant pBI121-NS1 plant expression vector provides a basis for the research on biological functions and applications of GPV NS1. |
| Keywords: | Goose parvovirus NS1 gene Agrobacterium LBA4404 Eukaryotic plant expressing vector |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《西北农业学报》浏览原始摘要信息 | |
| 点击此处可从《西北农业学报》下载免费的PDF全文 | |