Genetic investigation and cytological comparison of two genic male sterile lines 9012A and MSL in <Emphasis Type="Italic">Brassica napus</Emphasis> L. |
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| Authors: | Cancan Luo Yanyan Sun Yunxiao Zhang Yuan Guo Miroslav Klima Shengwu Hu |
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| Institution: | 1.State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy,Northwest A&F University,Yangling,China;2.Crop Research Institute,Prague 6,Czech Republic |
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| Abstract: | Genic male sterility (GMS) is one of the effective pollination control systems in hybrid rapeseed (Brassica napus L.) breeding. The recessive GMS lines 9012A and Lembke (MSL) have made a great contribution to the utilization of heterosis in rapeseed. Cytological observation and genetic investigation were employed to reveal anther abortion characters and genetic relationship of 9012A and MSL. Cytological and transmission electron microscopy observation revealed that anther abortion of 9012A and MSL initiated at the microsporocyte stage, both lines showed premature or retarded degradation of tapetum. Some anthers in 9012A developed to the microspore stage since their tapetal cells changed to the secretory type. However, MSL anthers did not progress to the tetrad stage, since the tapetum did not change to the secretory type. Genetic investigation revealed that MSL and 9012A had the same temporary maintainer system, while their cytoplasm types were different. The cytoplasm of MSL was similar to Cam while 9012A was similar to Nap type. The results laid a solid foundation for utilization of the two male sterile lines in rapeseed hybrid breeding. |
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