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Porcine salivary analysis by 2-dimensional gel electrophoresis in 3 models of acute stress: A pilot study |
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| Authors: | María Fuentes-Rubio José J Cerón Carlos de Torre Damián Escribano Ana M Gutiérrez Fernando Tecles |
| Institution: | Department of Animal Medicine and Surgery, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, 30100, Espinardo, Murcia, Spain (Fuentes-Rubio, Cerón, Escribano, Gutiérrez, Tecles); Proteomics Unit, University Hospital Virgen de la Arrixaca, Ctra Madrid-Cartagena s/n 30120, El Palmar-Murcia, Spain (de Torre). |
| Abstract: | The purpose of this research was to study changes in the salivary proteome of healthy pigs in stressful situations to identify any potential new salivary biomarker of stress. Three groups of animals were subjected to 3 stress models: snaring restraint followed by simulated sampling of vena cava blood; brief transport by road; and restriction of movement in a digestibility cage. Saliva was obtained from each animal before and 15 and 30 min after the induction of stress. The samples from the animals that showed the greatest increase in salivary cortisol concentration were pooled and run on 2-dimensional gels. Coomassie Brilliant Blue R-250 was used for spot detection and mass spectrometry for spot identification. Statistical analyses showed that 2 proteins had significant differences in expression before and after the induction of stress. These proteins were identified as odorant-binding protein and fragments of albumin. Further studies will be necessary to confirm the value of using these proteins as salivary biomarkers of stress in pigs. |
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