Evaluation of a real-time polymerase chain reaction assay of the outer membrane protein P2 gene for the detection of Haemophilus parasuis in clinical samples |
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| Authors: | Rebeccah McDowall Durda Slavic Janet I MacInnes Hugh Y Cai |
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| Institution: | Animal Health Laboratory, Laboratory Services Division, University of Guelph, 419 Gordon Street, Guelph, Ontario N1G 2W1 (McDowall, Slavic, Cai); Department of Pathobiology, University of Guelph, 419 Gordon Street, Guelph, Ontario N1G 2W1 (MacInnes). |
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| Abstract: | A real-time polymerase chain reaction (PCR) assay of the outer membrane protein (OMP) P2 gene was developed and used to test 97 putative Haemophilus parasuis pure cultures and 175 clinical tissue samples. With standard culture isolation as the gold standard, the diagnostic sensitivity and specificity of the PCR assay were determined to be 83% and 80%, respectively. |
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