| 黄芪中一种新的PR-10蛋白的纯化和性质 |
| |
| 引用本文： | 齐笑玮,闫巧娟,江正强,邓伟.黄芪中一种新的PR-10蛋白的纯化和性质[J].中国农业大学学报,2007,12(4):15-18. |
| |
| 作者姓名： | 齐笑玮 闫巧娟 江正强 邓伟 |
| |
| 作者单位： | 1. 中国农业大学,食品科学与营养工程学院,北京,100083
2. 中国农业大学,工学院,北京,100083 |
| |
| 摘 要： | 为了进一步研究蒙古黄芪(Astragalus mongholicusBunge)中的蛋白质及其功能,通过金属离子螯合层析、离子交换层析和凝胶过滤得到一种新蛋白(AmPR-10),并对其部分性质进行了研究。AmPR-10在SDS-PAGE上的分子质量为17.2 ku,凝胶过滤层析测得分子质量为32.6 ku,说明其为同源二聚体。糖蛋白染色显示为糖蛋白,总糖含量为13.7%,离子交换色谱法分析其中含有73%阿拉伯糖、15%葡萄糖、微量的果糖和一种未知糖;N-端序列分析结果显示其与病程相关蛋白家族PR-10中黄羽扇豆L1PR-10.1C同源性高达80%。同时,AmPR-10具有核糖核酸酶活性,纯AmPR-10的核糖核酸酶比活性为74.11 U/mg,这点与一些PR-10蛋白类似。
|
| 关 键 词： | 蒙古黄芪 病程相关蛋白PR-10 纯化 核糖核酸酶活性 |
| 文章编号： | 1007-4333（2007）04-0015-04 |
| 修稿时间： | 2006-12-26 |
Purification and characterization of a novel pathogenesis-related class-10 protein from Astragalus mongholicus |
| |
| Qi Xiaowei,Yan Qiaojuan,Jiang Zhengqiang,Deng Wei.Purification and characterization of a novel pathogenesis-related class-10 protein from Astragalus mongholicus[J].Journal of China Agricultural University,2007,12(4):15-18. |
| |
| Authors: | Qi Xiaowei Yan Qiaojuan Jiang Zhengqiang Deng Wei |
| |
| Institution: | 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China 2. College of Engineering, China Agricultural University, Beijing 100083, China |
| |
| Abstract: | A novel pathogenesis-related class-10 protein(AmPR-10) from Astragalus mongholicus was purified and characterized.The purified protein appeared as a single band with a molecular mass of 17.2 ku on SDS-PAGE and was eluted from a Superdex-75 column with an apparent molecular weight of 32.6 ku.AmPR-10 was a glycoprotein with a neutral carbohydrate content of 13.7%.The carbohydrate was mainly composed of 73.0% arabinose and 15.0% glucose.N-terminal amino-acid sequence of AmPR-10 was significantly homology to pathogenesis-related proteins from the PR-10 family.The sequence had 80% identity with L1PR10.1C,a PR-10 protein from Lupinus luteus.AmPR-10had ribonuclease activity of 74.11 U/mg protein,as some other PR-10 proteins. |
| |
| Keywords: | Astragalus mongholicus Bunge pathogenesis-related class10 proteins purification ribonuclease activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业大学学报》浏览原始摘要信息 |
| 点击此处可从《中国农业大学学报》下载免费的PDF全文 |
|
