| 假隔链格孢SF-193的产孢特性及其分生孢子对空心莲子草的致病力 |
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| 引用本文： | 聂亚锋,陈志谊,刘永锋,娄远来,刘邮洲,罗楚平,刘长河.假隔链格孢SF-193的产孢特性及其分生孢子对空心莲子草的致病力[J].中国生物防治,2009,25(3):260-266. |
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| 作者姓名： | 聂亚锋 陈志谊 刘永锋 娄远来 刘邮洲 罗楚平 刘长河 |
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| 作者单位： | 江苏省农业科学院植物保护研究所,南京,210014 |
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| 基金项目： | 江苏省高技术项目，国家科技支撑计划项目 |
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| 摘 要： | 为了探明SF-193的产孢特性,选取了8种产孢培养基,研究分别在自然光、黑光灯和黑暗的条件下对SF-193产孢的影响程度。试验结果表明,在黑光灯连续照射11d后,SF-193在PCA、CA、SNA、Czapek和组织煎汁5种培养基上有少量分生孢子产生,而在其他3种琼脂培养基上不产生分生孢子。PCA培养基中马铃薯与胡萝卜比例为1：5时,SF-193在9d后产孢量最大,10×10倍显微观察约60个孢子/视野;当CA培养基中的胡萝卜含量提高至80%左右时, SF-193在3d后达到最大产孢量,10×10倍显微观察约300个孢子/视野。研究了黑光灯照度与胡萝卜含量对SF-193产孢的影响。结果表明,在黑光灯照度为195～210lx、胡萝卜含量约为0.8g/ml时,SF-193在25℃培养6d后产孢量最大,约为6.5×10^5个孢子/ml。SF-193的分生孢子在田间对空心莲子草的致病力测定结果表明,经孢子浓度10^4个孢子/ml处理后,空心莲子草的病情指数为67.7。
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| 关 键 词： | 假隔链格孢SF-193 空心莲子草 产孢特性 致病力 |
Conidial Production of Nimbya alternantherae SF-193 and Its Pathogenicity to Alternanthera philoxeroides |
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| NIE Ya-feng
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CHEN Zhi-yi
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LIU Yong-feng
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LOU Yuan-lai
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LIU You-zhou
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LUO Chu-ping
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LIU Chang-he.Conidial Production of Nimbya alternantherae SF-193 and Its Pathogenicity to Alternanthera philoxeroides[J].Chinese Journal of Biological Control,2009,25(3):260-266. |
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| Authors: | NIE Ya-feng CHEN Zhi-yi LIU Yong-feng LOU Yuan-lai LIU You-zhou LUO Chu-ping LIU Chang-he |
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| Institution: | (Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) |
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| Abstract: | Nimbya alternantherae SF-193 was cultured on agar plates of eight media at 25℃under nature light,black light lamp and dark,respectively,for understanding its conidial production.The results showed that a few of conidia was produced on PCA,CA,SNA,Czapek and filtrate of the alligator weed under light from black light lamp for 11 d.And under natural light and dark,SF-193 could not produce conidia on these mediums.The maximum conidia yield was obtained on the PCA in irradiation of black light for 9 d when the proportion of potato and carrot was 1：5,and about 60 conidia were observed under magnification 10×10.However,about 300 conidia were observed on the medium of CA in irradiation of black light lamp for 3 d when the carrot content was increased to 80%.The intensity of illumination of the black light and carrot content could strongly influence conidia yield of SF-193.When the intensity of illumination of the black light lamp was about 195-210 lx and the carrot content of CA medium was increased to 80%,the amount of conidia was more than 6.5×10^5 conidia/ml after culturing for 6 d at 25℃.The pathogenicity of conidia to Alternanthera philoxeroides was tested in the field and the disease index of the weed was about 67.7. |
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| Keywords: | Nimbya alternantherae SF-193 Alternanthera philoxeroides characteristics of conidial production pathogenicity |
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