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| 壳聚糖对Bt发酵液的絮凝作用 | |
| 引用本文： | 张明政,刘波,林乃铨,阮传清,蓝江林.壳聚糖对Bt发酵液的絮凝作用[J].中国生物防治,2009,25(2):133-137. |
| 作者姓名： | 张明政 刘波 林乃铨 阮传清 蓝江林 |
| 作者单位： | 1. 福建省农业科学院农业生物资源研究所,福州350003;福建农林大学植物保护学院,福州350002 2. 福建省农业科学院农业生物资源研究所,福州,350003 3. 福建农林大学植物保护学院,福州,350002 |
| 基金项目： | 国家863计划，福建省星火计划 |
| 摘 要： | 为了将Bt发酵液高效浓缩,采用壳聚糖对Bt菌株LSZ9408发酵液进行絮凝,测定了pH值、温度、壳聚糖浓度、磁力搅拌的速度和时间对壳聚糖絮凝效果的影响。结果显示,当pH值为5.0～6.0,温度30～35℃,壳聚糖浓度为0.025%,磁力搅拌器550r/min搅拌5min后,壳聚糖作为LSZ9408菌株发酵液的絮凝剂,可除去发酵液中的大部分水及有色杂质,使发酵液中的杀虫蛋白质晶体和芽孢得到很好的浓缩。 |
| 关 键 词： | 壳聚糖 苏云金芽孢杆菌 LSZ9408菌株 发酵液 絮凝 浓缩 |
Factors Affecting Flocculation of Fermentation Broth of Bacillus thuringiensis by Chitosan |
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| ZHANG Ming-zheng , LIU Bo , LIN Nai-quan , RUAN Chuan-qing , LAN Jiang-lin.Factors Affecting Flocculation of Fermentation Broth of Bacillus thuringiensis by Chitosan[J].Chinese Journal of Biological Control,2009,25(2):133-137. | |
| Authors: | ZHANG Ming-zheng LIU Bo LIN Nai-quan RUAN Chuan-qing LAN Jiang-lin |
| Institution: | ZHANG Ming-zheng, LIU Bo, LIN Nai-quan, RUAN Chuan-qing, LAN Jiang-lin (1. Institute of Agro-Biological Resources, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China 2. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China ) |
| Abstract: | Chitosan was used as a flocculant to concentrate the active components from fermentation broth of Bacillus thuringiensis （Bt） LSZ9408. Chitosan dosage, rotation speed and rotation time of magnetic force stirrer, pH, and temperature for flocculation in the system were optimized. Results showed that a higher flocculation efficiency could be resulted in at pH 5.0 - 6.0, 30 - 35 ℃, ehitosan at 0. 025 %, and 5 min for stir at 550 r/min. Under the optimal conditions, most of water and tinct impurities were eliminated, and so the Bt insecticidal protein and spore was effectively concentrated. |
| Keywords: | chitosan Bt LSZ9408 fermentation broth flocculation condensation |
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